Dullaart R P, Groener J E, Dikkeschei B D, Erkelens D W, Doorenbos H
Department of Endocrinology, University Hospital, Groningen, Netherlands.
Diabetes Care. 1991 Apr;14(4):338-41. doi: 10.2337/diacare.14.4.338.
To determine the effect of cigarette smoking on the activity of cholesteryl ester transfer protein (CETP) and high-density (HDL), low-density (LDL), and very-low-density (VLDL) lipoproteins in insulin-dependent diabetic (IDDM) men with microvascular complications.
We performed a case-control study in a referral-based diabetes clinic on a sequential sample of 9 cigarette-smoking and 12 nonsmoking IDDM men with microvascular complications and 12 nonsmoking control men. CETP activity was determined in each serum with an isotope assay with exogenous cholesteryl ester-labeled LDL and HDL. The method is independent of the endogenous lipoprotein present in serum.
The HDL-cholesterol (VLDL and LDL) ratio was lower in the smoking diabetic men than in the other groups (P less than 0.05 vs. the nonsmoking diabetic men and P less than 0.01 vs. the control subjects). CETP activity was 70% higher in the smoking diabetic men than in the control subjects (P less than 0.01) and 30% higher than in the nonsmoking diabetic men (P less than 0.05). The HDL-cholesterol (VLDL and LDL) ratio and the apolipoprotein A-I-B ratio were inversely correlated to CETP activity in the diabetic patients (r = -0.52, P less than 0.02 and r = -0.45, P less than 0.05, respectively).
CETP activity is increased in cigarette-smoking IDDM men with microvascular complications. High CETP activity may contribute to the unfavorable lipoprotein profile in these patients.
确定吸烟对患有微血管并发症的胰岛素依赖型糖尿病（IDDM）男性患者中胆固醇酯转运蛋白（CETP）活性以及高密度脂蛋白（HDL）、低密度脂蛋白（LDL）和极低密度脂蛋白（VLDL）的影响。
我们在一家基于转诊的糖尿病诊所进行了一项病例对照研究，对9名吸烟且患有微血管并发症的IDDM男性、12名不吸烟且患有微血管并发症的IDDM男性以及12名不吸烟的对照男性进行了连续抽样。使用外源性胆固醇酯标记的LDL和HDL的同位素测定法测定每份血清中的CETP活性。该方法独立于血清中存在的内源性脂蛋白。
吸烟的糖尿病男性患者的HDL-胆固醇（VLDL和LDL）比值低于其他组（与不吸烟的糖尿病男性相比，P<0.05；与对照组相比，P<0.01）。吸烟的糖尿病男性患者的CETP活性比对照组高70%（P<0.01），比不吸烟的糖尿病男性患者高30%（P<0.05）。糖尿病患者中，HDL-胆固醇（VLDL和LDL）比值和载脂蛋白A-I-B比值与CETP活性呈负相关（r = -0.52，P<0.02；r = -0.45，P<0.05）。
患有微血管并发症的吸烟IDDM男性患者的CETP活性增加。高CETP活性可能导致这些患者出现不良的脂蛋白谱。
