Instituto de Inmunología, Universidad Nacional de Rosario, Rosario, Argentina.
Neuroimmunomodulation. 2011;18(1):11-8. doi: 10.1159/000313367. Epub 2010 Jul 7.
To study the effects of halothane anesthesia in mice not undergoing surgery on elements of the inflammatory and stress response; this involved assessment of the phagocytic activity and respiratory burst of peritoneal macrophages as well as plasma corticosterone levels and peripheral leukocyte counts.
There were 2 experimental groups, i.e. mice anesthetized with halothane 1.5% in oxygen for 40 min and a control group of mice subjected to the same manipulations but no anesthesia. At the end of the anesthetic or sham procedure, peritoneal macrophages were evaluated for phagocytic and lytic activity after an immune challenge and spontaneous respiratory burst (chemoluminiscence). Plasma corticosterone and leukocyte counts in peripheral blood were evaluated as indicators of the stress response.
In halothane-anesthetized mice, increased numbers and activity of phagocytic cells were found, with regard to the number of ingested and digested particles, compared to the nonanesthetized group. The ex vivo peritoneal macrophage respiratory burst without antigenic stimulation also showed a higher response in anesthetized mice compared with the nonanesthetized controls. Halothane administration did not alter corticosterone levels. Treated and control mice displayed similar leukocyte profiles in peripheral blood, except for lower lymphocyte counts in the controls compared to the halothane group. Typical correlation between corticosterone and leukocyte subsets, together with a high positive correlation between plasma corticosterone and phagocytic cell counts, were found only in the control group.
Halothane anesthesia might have beneficial effects on the inflammatory response mediated by phagocytes, namely the activity and efficiency of peritoneal macrophages, in a setting where plasma corticosterone and peripheral leukocyte counts were not affected.
研究未行手术的小鼠吸入卤烷麻醉对炎症和应激反应相关元素的影响；这包括评估腹腔巨噬细胞的吞噬活性和呼吸爆发、血浆皮质酮水平和外周白细胞计数。
有 2 个实验组，即接受 1.5%卤烷麻醉 40 分钟的小鼠和接受相同操作但不接受麻醉的对照组小鼠。在麻醉或假手术结束时，对腹腔巨噬细胞进行免疫挑战后吞噬和裂解活性以及自发呼吸爆发（化学发光）评估。外周血中的血浆皮质酮和白细胞计数作为应激反应的指标进行评估。
与未麻醉组相比，卤烷麻醉小鼠的吞噬细胞数量和活性增加，表现为摄入和消化的颗粒数量增加。在没有抗原刺激的情况下，离体腹腔巨噬细胞呼吸爆发在麻醉小鼠中也表现出更高的反应。卤烷给药并未改变皮质酮水平。与对照组相比，治疗组和对照组的外周血白细胞谱相似，但对照组的淋巴细胞计数较低。仅在对照组中发现皮质酮与白细胞亚群之间存在典型的相关性，以及血浆皮质酮与吞噬细胞计数之间的高度正相关。
在不影响血浆皮质酮和外周白细胞计数的情况下，卤烷麻醉可能对吞噬细胞介导的炎症反应产生有益影响，即腹腔巨噬细胞的活性和效率。
