Saki Ghasem, Rahim Fakher, Vaysi Ozra Allah
Laboratory of Cell Culture, Faculty of Medicine, Ahwaz.
J Hum Reprod Sci. 2010 Jan;3(1):32-4. doi: 10.4103/0974-1208.63120.
This study aimed to determine the effect of 50 days of forced swimming stress on fertilization capacity of rat and subsequent offspring quality.
The prospective study designed in vivo.
Total 90 Wistar rats including 30 adult male (3 months of age, weighing 210 +/- 10.6 g) and 60 female rats (3 months of age, weighing 230 +/- 12.2 g) were engaged in this study. Male rats were randomly divided in two equal groups (n = 15): Control and experimental groups. Animals of the experimental group were submitted to forced swimming stress for 3 min in water at 32 degrees C daily for 50 days. Then all adult male rats were mated with normal females (2 per each male) for 7 days. Female rats were sacrificed and autopsy was performed on day 20 of pregnancy when uterus and ovaries were examined for the number of corpora lutea, dead and live fetuses, embryo resorption, implantation sites, and fetus weight.
Results of this study have important implications for families attempting pregnancy. Stress pursuant to life events may have a negative impact on in vivo fertilization capacity of male rats and subsequent offspring quality.
本研究旨在确定50天强迫游泳应激对大鼠受精能力及后续子代质量的影响。
该前瞻性研究为体内实验设计。
本研究共纳入90只Wistar大鼠,其中包括30只成年雄性大鼠(3月龄,体重210±10.6 g)和60只雌性大鼠(3月龄,体重230±12.2 g)。雄性大鼠被随机分为两组,每组15只:对照组和实验组。实验组动物每天在32℃的水中进行3分钟的强迫游泳应激,持续50天。然后,所有成年雄性大鼠与正常雌性大鼠(每只雄性大鼠配2只雌性大鼠)交配7天。在妊娠第20天处死雌性大鼠并进行尸检,检查子宫和卵巢中的黄体数量、死胎和活胎数量、胚胎吸收情况、着床部位以及胎儿体重。
本研究结果对尝试怀孕的家庭具有重要意义。生活事件导致的应激可能会对雄性大鼠的体内受精能力及后续子代质量产生负面影响。