Hadzovic-Dzuvo Almira, Valjevac Amina, Avdagić Nesina, Lepara Orhan, Zaćiragić Asija, Jadrić Radivoj, Alajbegović Jasmin, Prnjavorac Besim
Department of Physiology, School of Medicine, University of Sarajevo, Bosnia and Herzegovina.
Med Glas (Zenica). 2011 Feb;8(1):121-5.
To estimate the effects of forced repeated swimming stress on BNP serum levels in rats.
Adult male Wistar rats weighting between 280-330 g were divided into two groups: control group (n = 8) and stress group (n = 8). Rats in the stress group were exposed to forced swimming stress daily, for 7 days. The rats were forced to swim in plastic tanks (90 cm wide, 120 cm deep) containing tap water (temperature ca. 25 degrees C). The depth of water was 40 cm. Duration of each swimming session progressively increased from 10 minutes on the first day to 40 minutes on days 6 and 7. Rats were sacrificed and blood was drawn from abdominal aorta for BNP analysis immediately after the last swimming session. B-type natriuretic serum level was determined by ELISA method using RAT BNP-32 kit (Phoenix Pharmaceutical Inc.).
There was no statistically significant difference between mean BNP serum level in the stress group after the swimming period (0.81 +/- 0.14 ng/ml) as compared to the unstressed group of rats (0.8 +/- 0.08 ng/ml). After the swimming period mean body weight slightly decreased in the stress group in comparison with values before stress period (296.3 g vs. 272.8 g), but this difference was not statistically significant. The stress period had no influence on food intake in the stress rat group.
The workload consisting of 40-minutes long swimming session is not sufficient to provoke BNP release from myocardium in rats.
评估强迫性重复游泳应激对大鼠血清脑钠肽（BNP）水平的影响。
将体重在280 - 330克之间的成年雄性Wistar大鼠分为两组：对照组（n = 8）和应激组（n = 8）。应激组大鼠每天接受强迫游泳应激，持续7天。大鼠被强迫在装有自来水（温度约25摄氏度）的塑料水箱（宽90厘米，深120厘米）中游泳。水深40厘米。每次游泳时间从第一天的10分钟逐渐增加到第6天和第7天的40分钟。在最后一次游泳结束后立即处死大鼠，从腹主动脉取血进行BNP分析。使用大鼠BNP - 32试剂盒（菲尼克斯制药公司）通过酶联免疫吸附测定法测定血清B型利钠肽水平。
与未应激的大鼠组（0.8 ± 0.08纳克/毫升）相比，游泳期后应激组大鼠的平均BNP血清水平（0.81 ± 0.14纳克/毫升）无统计学显著差异。与应激期前的值相比，应激组大鼠游泳期后的平均体重略有下降（296.3克对272.8克），但这种差异无统计学意义。应激期对应激大鼠组的食物摄入量没有影响。
由40分钟长的游泳组成的工作量不足以促使大鼠心肌释放BNP。
