Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Biol Trace Elem Res. 2011 Aug;142(2):167-73. doi: 10.1007/s12011-010-8756-4. Epub 2010 Jul 6.
The purpose of this 42-day study was to investigate the effects of low selenium (Se) on immune function by determining histopathological changes of thymus, apoptosis of thymic cells, and subpopulation of peripheral blood T cells. One hundred twenty 1-day-old avian broilers were randomly assigned to two groups of 60 each and were fed on a low Se diet (0.0342 mg/kg Se) or a control diet (0.2 mg/kg Se), respectively. The relative weight of thymus was significantly decreased in low Se group from 21 days of age in time-dependent manner when compared with that of control group. Histopathologically, lymphopenia in the cortex and medulla of thymus was observed in low Se group. In comparison with those of control group, the percentage of Annexin-V positive cells was increased, and the percentages of CD3(+) and CD3(+)CD8(+) T cells of the peripheral blood were decreased in low Se group, as measured by flow cytometry. These data suggested that low dietary Se induced histological lesions of thymus, increased apoptosis of thymic cells, and decreased T cell subsets. The cellular immune function was finally impaired in broilers.
本 42 天的研究旨在通过测定胸腺组织病理学变化、胸腺细胞凋亡和外周血 T 细胞亚群来研究低硒（Se）对免疫功能的影响。将 120 只 1 日龄禽肉鸡随机分为两组，每组 60 只，分别饲喂低硒（0.0342mg/kg Se）或对照（0.2mg/kg Se）日粮。与对照组相比，低 Se 组从 21 日龄开始，胸腺相对重量呈时间依赖性下降。组织病理学观察发现，低 Se 组胸腺皮质和髓质淋巴细胞减少。与对照组相比，流式细胞术检测显示，低 Se 组外周血 Annexin-V 阳性细胞的百分比增加，CD3(+)和 CD3(+)CD8(+)T 细胞的百分比降低。这些数据表明，低膳食 Se 可诱导胸腺组织病理学损伤，增加胸腺细胞凋亡，减少 T 细胞亚群。最终导致肉鸡细胞免疫功能受损。
