Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan, China, 625014.
Biol Trace Elem Res. 2011 Nov;143(2):932-8. doi: 10.1007/s12011-010-8890-z. Epub 2010 Nov 3.
The purpose of this 42-day study was to investigate the effects of dietary excess vanadium on immune function by determining changes of the subsets and proliferation function of splenic T cells. Four hundred twenty 1-day-old avian broilers were divided into six groups and fed on a corn-soybean basal diet as control diet or the same diet amended to contain 5, 15, 30, 45, and 60 ppm of vanadium supplied as ammonium metavanadate. When compared with those of the control group, the percentage of CD3+, CD3+CD4+, and CD3+CD8+ of splenic T cells were decreased in the 45 and 60 ppm groups; however, the percentage of CD3+ and CD3+CD4+ were increased in the 5 ppm group, and the CD4+/CD8+ ratios were raised in the 5 and 15 ppm groups at 14 days of age. Meanwhile, the proliferation of splenic T cells were depressed in the 45 and 60 ppm groups but raised in the 5 and 15 ppm groups. Also, the serum interleukin-2 (IL-2) and interleukin-6 (IL-6) contents were decreased in the 45 and 60 ppm groups and increased in the 5 ppm group. It was concluded that dietary vanadium in excess of 30 ppm changed the percentages of splenic T cell subsets and inhibited the proliferation of splenic T cells and reduced the serum IL-2 and IL-6 contents. The cellular immune function was finally impaired in broilers.
本 42 天的研究旨在通过测定脾 T 细胞亚群和增殖功能的变化来研究膳食过量钒对免疫功能的影响。将 420 只 1 日龄禽肉鸡分为 6 组，分别用玉米-大豆基础日粮作为对照日粮或相同日粮进行喂养，日粮中添加 5、15、30、45 和 60ppm 的偏钒酸铵作为钒源。与对照组相比，45 和 60ppm 组脾 T 细胞中 CD3+、CD3+CD4+和 CD3+CD8+的百分比降低；然而，在 5ppm 组中，CD3+和 CD3+CD4+的百分比增加，在 5 和 15ppm 组中，CD4+/CD8+的比值在 14 天龄时升高。同时，45 和 60ppm 组脾 T 细胞的增殖受到抑制，但在 5 和 15ppm 组中得到提高。此外，45 和 60ppm 组血清白细胞介素-2（IL-2）和白细胞介素-6（IL-6）含量降低，5ppm 组含量增加。结果表明，日粮中过量的钒（超过 30ppm）改变了脾 T 细胞亚群的百分比，抑制了脾 T 细胞的增殖，并降低了血清 IL-2 和 IL-6 的含量。最终，肉鸡的细胞免疫功能受到损害。
