College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, China, 625014.
Biol Trace Elem Res. 2011 Dec;144(1-3):688-94. doi: 10.1007/s12011-011-9077-y. Epub 2011 May 21.
The purpose of this 42-day study was to investigate the effects of low selenium (Se) on cellular immune function by determining cell cycle of thymus, serum IL-2 content, and mitogenesis of peripheral blood T-lymphocytes. One hundred twenty 1-day-old Avian broilers were randomly assigned to two groups of 60 each and were fed on a low-Se diet (0.0342 mg/kg Se) or a control diet (0.2 mg/kg Se), respectively. Cell cycle analysis by flow cytometry showed that low-Se diet caused an increase in G(0)G(1) phase cells that corresponded to a decrease in S-phase cells in thymus. Ultrastructurally, mitochondria injury and increased apoptotic cells with condensed nuclei were observed. Low-Se diet decreased the serum IL-2 contents and mitogenesis of peripheral blood lymphocytes to concanavalin A in comparison with those of control group. These data indicate that low-Se diet inhibits the development of thymus by arresting the cell cycle and decreasing the IL-2 content.
本 42 天研究旨在通过测定胸腺细胞周期、血清 IL-2 含量和外周血 T 淋巴细胞有丝分裂来研究低硒（Se）对细胞免疫功能的影响。将 120 只 1 日龄肉鸡随机分为两组，每组 60 只，分别饲喂低硒（0.0342mg/kg Se）或对照（0.2mg/kg Se）饮食。流式细胞术分析细胞周期显示，低硒饮食导致胸腺 G0/G1 期细胞增加，S 期细胞减少。超微结构观察到线粒体损伤和核固缩的凋亡细胞增加。与对照组相比，低硒饮食降低了血清 IL-2 含量和外周血淋巴细胞对刀豆蛋白 A 的有丝分裂。这些数据表明，低硒饮食通过阻断细胞周期和降低 IL-2 含量来抑制胸腺发育。
