Ogasawara J, Kamata Y, Sakaguchi G, Kozaki S
Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Japan.
FEMS Microbiol Lett. 1991 Apr 15;63(2-3):351-5. doi: 10.1016/0378-1097(91)90111-m.
To characterize an acceptor for Clostridium botulinum type B neurotoxin, its binding kinetics were examined with mouse brain synaptosomes treated with various enzymes. The amount of 125I-labelled neurotoxin bound to synaptosomes decreased upon treatment with lysyl endopeptidase, neuraminidase, or phospholipase C. The binding of the neurotoxin was partially recovered by incubation of neuraminidase-treated synaptosomes with ganglioside GT1b or GD1a. Gangliosides incorporated into untreated, lysyl endopeptidase-treated, and phospholipase C-treated synaptosomes had no effect on the binding of the neurotoxin. These results may suggest that type B neurotoxin binds to gangliosides in cooperation with a certain protease-sensitive substance on the neural membranes.
