Hirokawa N, Kitamura M
J Cell Biol. 1979 Apr;81(1):43-9. doi: 10.1083/jcb.81.1.43.
Large synaptosome fractions were isolated from the cerebellar and cerebral cortices of rats and were incubated with Clostridium botulinum type A neurotoxin in vitro. The binding of the neurotoxin to the synapses was observed by electron microscopy, using the double-sandwich immunocytochemical method. Botulinum neurotoxin was preferentially bound to the presynaptic membrane in the large synaptosome fraction. The binding regions for the neurotoxin were localized on both the extrajunctional and junctional areas of the presynaptic membranes and appeared as patches of various sizes. However, they did not exist on the postsynaptic membranes. Botulinum neurotoxin is proposed to be a useful analytical tool for understanding the characteristics of the presynaptic membranes in the central nervous system.
从大鼠的小脑和大脑皮层中分离出大的突触体组分,并在体外与A型肉毒杆菌神经毒素一起孵育。使用双夹心免疫细胞化学方法,通过电子显微镜观察神经毒素与突触的结合情况。肉毒杆菌神经毒素优先结合大突触体组分中的突触前膜。神经毒素的结合区域定位于突触前膜的结外和结区,呈现为各种大小的斑块。然而,它们不存在于突触后膜上。肉毒杆菌神经毒素被认为是一种用于了解中枢神经系统突触前膜特征的有用分析工具。