L'vov D K, Iashkulov K B, Prilipov A G, Burtseva E I, Shchelkanov M Iu, Shliapnikova O V, Poglazov A B, Sadykova G K, Dzhambinov S D, Fediakina I T, Bushkieva B Ts, L'vov D N, Zhuravleva M M, Al'khovskiĭ S V, Samokhvalov E I, Trushakova S V, Lavrishcheva V V, Vereshchagin N N, Mikhaliaeva L B, Darbakova T A, Limanskaia O S, Dzhaparidze N I, Imkenova L N, Ledenev Iu A, Boldyreva V V, Ivanov L I, Zdanovskaia N N
Vopr Virusol. 2010 May-Jun;55(3):15-8.
The paper analyzes the amino acid sequence of the receptor-binding site of hemagglutinin (HA) in the variants of pandemic influenza A/H1N1 swl from 18 patients with moderate (n=1) and fatal (n=17) forms of the disease in 2009. Nine samples contained asparaginic acid at position 222 of HA1 (D). This site exhibited mutations in 9 samples: D222G (n=3), D222N (n=3), and D222G/D222N (n=3). In one patient with the moderate form of the disease, D222G mutation was revealed after the second passage in the developing chick embryos; this mutation was not found in the primary sample from the patient. The findings suggest the mutant variants of the virus start to circulate among the population, which requires, firstly, continuation of molecular virological monitoring of the pandemic situation and, secondly, further study of the impact of amino acid substitutions at the receptor-binding site of HA1 on the increased virulence of influenza A virus.
该论文分析了2009年18例患有中度（n = 1）和致命（n = 17）形式疾病的甲型H1N1流感大流行病毒株血凝素（HA）受体结合位点的氨基酸序列。九个样本在HA1的第222位含有天冬氨酸（D）。该位点在9个样本中出现突变：D222G（n = 3）、D222N（n = 3）和D222G/D222N（n = 3）。在一名患有中度疾病的患者中，在发育中的鸡胚中传代两次后发现了D222G突变；在该患者的原始样本中未发现此突变。研究结果表明，病毒的突变变体开始在人群中传播，这首先需要继续对大流行情况进行分子病毒学监测，其次需要进一步研究HA1受体结合位点的氨基酸替换对甲型流感病毒毒力增加的影响。
