Provincial Laboratory for Public Health Microbiology, Calgary Site, Calgary, AB, Canada.
Clin Microbiol Infect. 2011 Apr;17(4):582-4. doi: 10.1111/j.1469-0691.2010.03341.x. Epub 2010 Nov 5.
Pandemic (H1N1) 2009 virus-positive specimens were collected from autopsy patients and matched to pandemic (H1N1) 2009 virus-positive nasopharyngeal specimens from community control patients and pandemic (H1N1) 2009 virus-positive specimens from intensive-care unit (ICU) patients. Specimens were analysed for polymorphisms at amino acid 222 of the haemagglutinin (HA) glycoprotein. Whereas some specimens from autopsy patients were positive for D222N, none was positive for D222G. All control patient specimens were wild-type D222. D222G polymorphisms were also identified in a subset of ICU patients with admixtures of D222G and D222 and of D222N, D222G and D222 present. The relevance of D222N and D222G to influenza pathogenesis and transmissibility currently remains unclear.
从尸检患者中采集了甲型 H1N1 流感病毒阳性标本，并与社区对照患者的甲型 H1N1 流感病毒阳性鼻咽标本和重症监护病房（ICU）患者的甲型 H1N1 流感病毒阳性标本进行了匹配。对血凝素（HA）糖蛋白 222 位氨基酸的多态性进行了分析。虽然一些尸检患者的标本呈 D222N 阳性，但没有标本呈 D222G 阳性。所有对照患者的标本均为野生型 D222。在 ICU 患者的亚组中也发现了 D222G 多态性，其中存在 D222G 和 D222 的混合物，以及 D222N、D222G 和 D222 的混合物。D222N 和 D222G 与流感发病机制和传染性的相关性目前尚不清楚。
