Centro Nacional de Gripe-Madrid, Instituto de Salud Carlos III, Madrid, Spain.
J Clin Virol. 2011 May;51(1):75-8. doi: 10.1016/j.jcv.2011.01.020. Epub 2011 Feb 24.
A change of aspartic acid (D) to glycine (G) at position 222 in the haemagglutinin (HA) protein of pandemic influenza A (H1N1) 2009 viruses was described in Norway on November 2009 with considerable frequency in fatal and severe cases. This change was detected in other countries and was related only with severe disease. Other substitutions to glutamic acid (E) or asparagine (N) at position 222 were detected among pandemic viruses but it is unclear what implications might have in terms of severity.
To analyse the appearance of amino acid substitutions at position 222 in the HA protein of circulating viruses in Spain and to determine their relationships with the disease symptoms observed.
Pandemic influenza A (H1N1) 2009 viruses detected in respiratory samples of 273 severe and 533 non-severe cases from different Spanish regions were selected for sequencing of a partial segment of HA1 subunit and studied to monitor substitutions at position 222.
D222G substitution was only detected in viruses from 14 severe cases (5.12%). D222E was found in viruses from 47 severe (17.21%) and from 52 non-severe cases (9.75%). D222N occurred in viruses from 3 additional severe cases (0.37%).
Appearance of D222G and D222E substitution in HA of pandemic influenza A (H1N1) viruses circulating in Spain might be related with severe respiratory disease.
2009 年 11 月，在挪威发现，大流行性甲型流感（H1N1）2009 病毒血凝素（HA）蛋白第 222 位天冬氨酸（D）突变为甘氨酸（G），在重症和致死病例中相当频繁。在其他国家也检测到了这种变化，且仅与严重疾病有关。在大流行病毒中还检测到其他位置的谷氨酸（E）或天冬酰胺（N）取代 222，但尚不清楚这在严重程度方面可能有何影响。
分析西班牙流行病毒 HA 蛋白第 222 位氨基酸取代的出现情况，并确定其与观察到的疾病症状之间的关系。
选择来自西班牙不同地区的 273 例重症和 533 例非重症呼吸道样本中检测到的大流行性甲型流感（H1N1）2009 病毒进行部分 HA1 亚单位测序，并研究 222 位的取代情况。
仅在 14 例重症病例（5.12%）的病毒中检测到 D222G 取代。在 47 例重症（17.21%）和 52 例非重症病例（9.75%）的病毒中发现了 D222E。在另外 3 例重症病例（0.37%）的病毒中发现了 D222N。
在西班牙流行的甲型流感（H1N1）病毒 HA 中出现 D222G 和 D222E 取代可能与严重呼吸道疾病有关。
