Ozbek Emin, Ilbey Y Yusuf Ozlem, Simşek Abdulmuttalip, Cekmen Mustafa, Balbay M Derya
Istanbul-Vakif Gureba Research & Education Hospital, Department of Urology, Fatih-Istanbul, Turkey.
Arch Ital Urol Androl. 2009 Dec;81(4):248-50.
In vitro studies have shown that nitric oxide (NO), inhibits sperm motility at high concentrations. In this study we aimed to determine the NO levels in the seminal fluid of patients with infertile varicocele both pre and postoperatively and in the control group, and compare the results. 20 men with varicocele presented to our clinic for primary infertility and 15 normal fertile men as controls were involved to study. NO levels in the seminal fluid were determined as the total nitrite by Griess reaction and results were compared with Mann-Whitney U test. Preoperative and postoperative mean seminal fluid NO levels in patients with varicocele were 114.82 +/- 33.02 micromol/L and 93.17 +/- 27.24 micromol/L, respectively. In the control group it was 89.4 +/- 20.82 micromol/L. There was a statistically significant different between mean preoperative and postoperative seminal NO levels (p < 0.05), whereas there was no significant difference between mean postoperative seminal NO levels and that of control group (p > 0.05). According to the results of present study, an increase in the level of seminal NO levels may play a role in the sperm dysfunction in infertile patients with varicocele.
体外研究表明，高浓度的一氧化氮（NO）会抑制精子活力。在本研究中，我们旨在测定不育精索静脉曲张患者术前和术后以及对照组精液中的NO水平，并比较结果。20名因原发性不育前来我们诊所就诊的精索静脉曲张男性和15名正常生育男性作为对照参与了研究。通过格里斯反应将精液中的NO水平测定为总亚硝酸盐，并将结果用曼-惠特尼U检验进行比较。精索静脉曲张患者术前和术后精液中NO的平均水平分别为114.82±33.02微摩尔/升和93.17±27.24微摩尔/升。对照组为89.4±20.82微摩尔/升。术前和术后精液中NO的平均水平之间存在统计学显著差异（p<0.05），而术后精液中NO的平均水平与对照组之间无显著差异（p>0.05）。根据本研究结果，精液中NO水平的升高可能在不育精索静脉曲张患者的精子功能障碍中起作用。
