Department of Chemistry, Yale University, New Haven, CT 06520-8107, USA.
Biochemistry. 2010 Aug 3;49(30):6302-4. doi: 10.1021/bi100976w.
The discrimination of n-alkyl-saturated aldehydes during the early stage of odorant recognition by the rat I7 olfactory receptor (OR-I7) is investigated. The concentrations of odorants necessary for 50% activation (or inhibition) of the OR-I7 are measured by calcium imaging recordings of dissociated rat olfactory sensory neurons, expressing the recombinant OR-I7 from an adenoviral vector. These are correlated with the corresponding binding free energies computed for a homology structural model of the OR-I7 built from the crystal structure of bovine visual rhodopsin at 2.2 A resolution.
研究了大鼠 I7 嗅觉受体 (OR-I7) 在嗅觉识别早期对 n-烷基饱和醛的识别能力。通过表达重组 OR-I7 的腺病毒载体的分离大鼠嗅觉感觉神经元的钙成像记录，测量了使 OR-I7 激活（或抑制）达到 50%所需的浓度。这些与相应的结合自由能相关联，这些自由能是通过同源结构模型计算得出的，该模型是根据 2.2A 分辨率的牛视觉视紫红质晶体结构构建的。
