评价化学消毒剂对热聚合丙烯酸树脂消毒效果的研究。
Evaluation of the efficacy of chemical disinfectants for disinfection of heat-polymerised acrylic resin.
机构信息
Fixed Partial Dentures, Department of Dental Materials and Prosthodontics, Dental School of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
出版信息
Gerodontology. 2011 Dec;28(4):253-7. doi: 10.1111/j.1741-2358.2010.00400.x. Epub 2010 Jul 1.
OBJECTIVE
This study evaluated the efficacy of disinfectants on the internal aspect of heat-polymerised acrylic resin contaminated with microbial strains.
BACKGROUND
Dentures absorb oral fluids and become contaminated by different microorganisms.
METHODS
Two hundred and fifty rectangular specimens were made of heat-polymerised acrylic resin, and then divided into five groups corresponding to the microbial strains (Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, S. mutans and Enterococcus faecalis). After contamination, the specimens were immersed in 1 and 2% sodium hypochlorite and 2% glutaraldehyde for periods of 5, 10 and 15 min. The specimens were placed into tubes containing different broths and incubated at 35°C and then visually analysed. Turbidity in the medium indicated microbial growth. The Fisher's exact test was used in the analysis of the results.
RESULTS
The strain E. faecalis was the most resistant to the disinfectant solutions, and among them, glutaraldehyde was more effective than 2 and 1% hypochlorite for disinfection for 5 min; in the 10-min period there were no differences between the disinfectants. In 15 min of immersion, 1% hypochlorite and glutaraldehyde were more effective than 2% hypochlorite.
CONCLUSIONS
Disinfection for 10 min with 1% hypochlorite and glutaraldehyde is effective in disinfecting the internal aspect of heat-polymerised acrylic resin.
目的
本研究评估了消毒剂对热聚合丙烯酸树脂内部受微生物菌株污染的效果。
背景
义齿吸收口腔液体并被不同的微生物污染。
方法
用热聚合丙烯酸树脂制作 250 个矩形样本，然后将其分为 5 组，对应于微生物菌株（金黄色葡萄球菌、铜绿假单胞菌、白色念珠菌、变形链球菌和粪肠球菌）。污染后，将标本浸泡在 1%和 2%次氯酸钠和 2%戊二醛中 5、10 和 15 分钟。将标本放入含有不同肉汤的试管中，在 35°C 下孵育，然后进行目视分析。培养基中的浑浊表明微生物生长。Fisher 确切检验用于分析结果。
结果
菌株 E. faecalis 对消毒剂溶液的抵抗力最强，其中戊二醛在 5 分钟的消毒时间内比 2%和 1%次氯酸钠更有效；在 10 分钟的时间内，消毒剂之间没有差异。在 15 分钟的浸泡时间内，1%次氯酸钠和戊二醛比 2%次氯酸钠更有效。
结论
用 1%次氯酸钠和戊二醛消毒 10 分钟可有效消毒热聚合丙烯酸树脂的内部。
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