Silva Matheus Jacobina, de Oliveira Denise G, Marcillo Oscar O, Neppelenbroek Karin H, Lara Vanessa S, Porto Vinícius C
Department of Prosthodontics, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
Department of Stomatology, Bauru Dental School, University of São Paulo, Bauru, São Paulo, Brazil.
Int Dent J. 2016 Apr;66(2):86-92. doi: 10.1111/idj.12212. Epub 2016 Jan 7.
Denture stomatitis is the most common pathology affecting denture wearers and its main cause is colonisation of dentures with Candida albicans.
This study investigated the effectiveness of two commercial composite surface sealants (Biscover(®) LV and Surface Coat(®)) to reduce C. albicans biofilm colonisation on denture resin, as well as their surface integrity after disinfection cycles with 1% sodium hypochlorite solution.
Heat-cured acrylic resin specimens were manufactured (10 mm × 10 mm × 1 mm). The specimen surfaces were mechanically polished to simulate rough or smooth denture surfaces. Four surface-treatment groups were tested: smooth surfaces [0.3 μm of mean roughness (Ra)]; rough surfaces (3 μm of Ra); rough surfaces treated with Biscover(®) LV; and rough surfaces treated with Surface Coat(®). Specimens of each group were randomly divided to undergo immersion in distilled water or 1% sodium hypochlorite for 30 or 90 cycles each. Specimens of all groups in each immersion solution were tested using a crystal violet (CV) staining assay for biofilm quantification and by scanning electron microscopy for visual analyses of surface integrity and biofilm structure. CV assay data were analysed using one-way analysis of variance (ANOVA) followed by Tukey's multiple comparison test (P < 0.05).
The effectiveness and surface integrity of Biscover(®) LV-treated surfaces were similar to those of smooth surfaces, whereas Surface Coat(®) -treated surfaces presented a similar performance to rough surfaces in all solutions and cycles.
These results suggest the possibility of clinical use of Biscover(®) LV for denture coating on surfaces in which mechanical polish is not indicated, such as the fitting surface.
义齿性口炎是影响义齿佩戴者的最常见病理状况，其主要原因是义齿被白色念珠菌定植。
本研究调查了两种商用复合表面密封剂（Biscover® LV和Surface Coat®）减少白色念珠菌生物膜在义齿树脂上定植的有效性，以及它们在1%次氯酸钠溶液消毒循环后的表面完整性。
制作热固化丙烯酸树脂标本（10毫米×10毫米×1毫米）。对标本表面进行机械抛光以模拟粗糙或光滑的义齿表面。测试四个表面处理组：光滑表面[平均粗糙度（Ra）为0.3微米]；粗糙表面（Ra为3微米）；用Biscover® LV处理的粗糙表面；以及用Surface Coat®处理的粗糙表面。每组标本随机分为在蒸馏水中或1%次氯酸钠中浸泡，每种浸泡30或90个循环。使用结晶紫（CV）染色试验对每种浸泡溶液中所有组的标本进行生物膜定量测试，并通过扫描电子显微镜对表面完整性和生物膜结构进行视觉分析。使用单因素方差分析（ANOVA），然后进行Tukey多重比较检验分析CV试验数据（P < 0.05）。
Biscover® LV处理表面的有效性和表面完整性与光滑表面相似，而Surface Coat®处理表面在所有溶液和循环中表现与粗糙表面相似。
这些结果表明，对于诸如贴合面等不适合进行机械抛光的表面，Biscover® LV有可能临床用于义齿涂层。
