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miRNA-106b 在传统肾细胞癌中的表达是预测肾切除术后早期转移的潜在标志物。

Expression of miRNA-106b in conventional renal cell carcinoma is a potential marker for prediction of early metastasis after nephrectomy.

机构信息

Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Zluty kopec 7, Brno, Czech Republic.

出版信息

J Exp Clin Cancer Res. 2010 Jul 7;29(1):90. doi: 10.1186/1756-9966-29-90.

DOI:10.1186/1756-9966-29-90
PMID:20609231
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2907341/
Abstract

BACKGROUND

MicroRNAs are endogenously expressed regulatory noncoding RNAs. Previous studies have shown altered expression levels of several microRNAs in renal cell carcinoma.

METHODS

We examined the expression levels of selected microRNAs in 38 samples of conventional renal cell carcinoma (RCC) and 10 samples of non-tumoral renal parenchyma using TaqMan real-time PCR method.

RESULTS

The expression levels of miRNA-155 (p < 0.0001), miRNA-210 (p < 0.0001), miRNA-106a (p < 0.0001) and miRNA-106b (p < 0.0001) were significantly over-expressed in tumor tissue, whereas the expression of miRNA-141 (p < 0.0001) and miRNA-200c (p < 0.0001) were significantly decreased in RCC samples. There were no significant differences between expression levels of miRNA-182 and miRNA-200b in tumor samples and renal parenchyma. Our data suggest that expression levels of miRNA-106b are significantly lower in tumors of patients who developed metastasis (p = 0.030) and miR-106b is a potential predictive marker of early metastasis after nephrectomy in RCC patients (long-rank p = 0.032).

CONCLUSIONS

We have confirmed previous observations obtained by miRNA microarray analysis using standardized real-time PCR method. For the first time, we have identified a prognostic significance of miRNA-106b, which, after validation on a larger group of patients, maybe useful as a promising biomarker in patients with RCC.

摘要

背景

microRNAs 是内源性表达的调节性非编码 RNA。先前的研究表明，几种 microRNAs 在肾细胞癌中的表达水平发生了改变。

方法

我们使用 TaqMan 实时 PCR 方法检测了 38 例常规肾细胞癌（RCC）和 10 例非肿瘤性肾实质样本中选定 microRNAs 的表达水平。

结果

miRNA-155（p<0.0001）、miRNA-210（p<0.0001）、miRNA-106a（p<0.0001）和 miRNA-106b（p<0.0001）的表达水平在肿瘤组织中显著上调，而 miRNA-141（p<0.0001）和 miRNA-200c（p<0.0001）的表达在 RCC 样本中显著下调。miRNA-182 和 miRNA-200b 在肿瘤样本和肾实质中的表达水平没有显著差异。我们的数据表明，在发生转移的患者肿瘤中 miRNA-106b 的表达水平显著降低（p=0.030），miR-106b 是 RCC 患者肾切除术后早期转移的潜在预测标志物（long-rank p=0.032）。

结论

我们使用标准化实时 PCR 方法证实了以前使用 miRNA 微阵列分析获得的观察结果。我们首次确定了 miRNA-106b 的预后意义，在对更大患者群体进行验证后，它可能成为 RCC 患者有前途的生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e127/2907341/b42c3502c3d3/1756-9966-29-90-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e127/2907341/4643e6ed9aff/1756-9966-29-90-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e127/2907341/e9b70dcfdebe/1756-9966-29-90-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e127/2907341/f7b5bf58a195/1756-9966-29-90-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e127/2907341/b42c3502c3d3/1756-9966-29-90-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e127/2907341/4643e6ed9aff/1756-9966-29-90-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e127/2907341/e9b70dcfdebe/1756-9966-29-90-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e127/2907341/f7b5bf58a195/1756-9966-29-90-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e127/2907341/b42c3502c3d3/1756-9966-29-90-4.jpg

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2
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3
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Int J Mol Sci. 2023 Apr 7;24(8):6909. doi: 10.3390/ijms24086909.
4
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Transl Cancer Res. 2020 Sep;9(9):5798-5818. doi: 10.21037/tcr-20-1294.
5
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7
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9
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5
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7
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J Urol. 2010 Feb;183(2):743-51. doi: 10.1016/j.juro.2009.09.086.
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