急性心肌梗死中新型分子网络表达的鉴定
Identification of Novel Molecular Network Expression in Acute Myocardial Infarction.
作者信息
Matboli Marwa, Shafei Ayman E, Agwa Sara H A, Elzahy Sherif Sammir, Anwar Ahmed K, Mansour Amr R, Gaber Ahmed I, Said Ali E A, Lwis Paula, Hamdy Marwa
机构信息
Medicinal Biochemistry and Molecular Biology Department, Ain Shams University, Faculty of Medicine, Cairo, Egypt.
Biomedical Research Department, Military Medical Academy, Cairo, Egypt.
出版信息
Curr Genomics. 2019 Aug;20(5):340-348. doi: 10.2174/1389202920666190820142043.
BACKGROUND
In the current study, we aimed to analyze the hypothesis that human myocardial-specific extracellular RNAs expression could be used for acute myocardial injury(AMI) diagnosis.
METHODOLOGY
We used bioinformatics' analysis to identify RNAs linked to ubiquitin system and specific to AMI, named, (lncRNA-RP11-175K6.1), (LOC101927740), microRNA-106b-5p (miR-106b-5p) and Anaphase, promoting complex 11 (). We measured the serum expression of the chosen RNAs in 69 individuals with acute coronary syndromes, 31 individuals with angina pectoris without MI and non-cardiac chest pain and 31 healthy control individuals by real-time reverse-transcription PCR.
RESULTS
Our study revealed a significant decrease in both lncRNA-RP11-175K6. and expression of in the sera samples of AMI patients compared to that of the two control groups alongside with significant upregulation of .
CONCLUSION
Of note, the investigated serum RNAs decrease the false discovery rate of AMI to 3.2%.
背景
在本研究中，我们旨在分析关于人心肌特异性细胞外RNA表达可用于急性心肌损伤（AMI）诊断这一假说。
方法
我们运用生物信息学分析来鉴定与泛素系统相关且对AMI具有特异性的RNA，分别命名为（lncRNA-RP11-175K6.1）、（LOC101927740）、微小RNA-106b-5p（miR-106b-5p）和后期促进复合体11（）。我们通过实时逆转录聚合酶链反应测量了69例急性冠脉综合征患者、31例无心肌梗死的心绞痛患者及非心源性胸痛患者以及31例健康对照个体血清中所选RNA的表达情况。
结果
我们的研究显示，与两个对照组相比，AMI患者血清样本中的lncRNA-RP11-175K6.1和 的表达均显著降低，同时 显著上调。
结论
值得注意的是，所研究的血清RNA将AMI的假发现率降低至3.2%。
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