College of Life Sciences, Capital Normal University, Beijing 100048, People's Republic of China.
J Agric Food Chem. 2010 Aug 11;58(15):8490-4. doi: 10.1021/jf100598k.
As more and more genetically modified organisms (GMO) are commercialized, efficient and inexpensive assays are required for their quick detection. An event-specific detection strategy based on the unique and specific sequences of integration junctions is useful because of its high specificity. This study developed a system for detecting six GM maize lines (Bt11, Bt176, GA21, MON810, NK603, and T25) using optical silicon thin-film biosensor chips. Aldehyde-labeled probes were arrayed and covalently attached to a hydrazine-derivatized chip surface. Biotinylated PCR amplicons were then hybridized with the probes. After washing and brief incubation with an anti-biotin IgG horseradish peroxidase conjugate and a precipitable horseradish peroxidase substrate, biotinylated PCR amplicons perfectly matched with the probes can be visualized by the color change on the chip surface (gold to blue/purple). This assay is extremely robust, exhibits high sensitivity and specificity, and is flexible from low through moderate to high throughput.
随着越来越多的转基因生物（GMO）被商业化，需要快速检测它们，因此需要高效、廉价的检测方法。基于整合连接点独特和特异性序列的特定事件检测策略因其高特异性而非常有用。本研究开发了一种使用光学硅薄膜生物传感器芯片检测六种转基因玉米品系（Bt11、Bt176、GA21、MON810、NK603 和 T25）的系统。醛基标记的探针被排列并共价连接到肼衍生的芯片表面。然后，生物素化的 PCR 扩增子与探针杂交。洗涤后，与抗生物素 IgG 辣根过氧化物酶缀合物和可沉淀的辣根过氧化物酶底物短暂孵育后，与探针完全匹配的生物素化 PCR 扩增子可以通过芯片表面的颜色变化（金变蓝/紫）来可视化。该检测方法非常稳健，具有高灵敏度和特异性，并且从低通量到中通量再到高通量都具有灵活性。
