Bass Chris, Nikou Dimitra, Vontas John, Donnelly Martin J, Williamson Martin S, Field Linda M
Department of Biological Chemistry, Rothamsted Research, Harpenden AL5 2JQ, UK.
Malar Res Treat. 2010;2010:190434. doi: 10.4061/2010/190434. Epub 2010 Dec 22.
Regular monitoring of mosquito vector populations is an integral component of most vector control programmes. Contemporary data on mosquito species composition, infection status, and resistance to insecticides are a prerequisite for effective intervention. For this purpose we, with funding from the Innovative Vector Control Consortium (IVCC), have developed a suite of high-throughput assays based on a single "closed-tube" platform that collectively comprise the "Vector Population Monitoring Tool" (VPMT). The VPMT can be used to screen mosquito disease vector populations for a number of traits including Anopheles gambiae s.l. and Anopheles funestus species identification, detection of infection with Plasmodium parasites, and identification of insecticide resistance mechanisms. In this paper we focus on the Anopheles-specific assays that comprise the VPMT and include details of a new assay for resistance todieldrin Rdl detection. The application of these tools, general and specific guidelines on their use based on field testing in Africa, and plans for further development are discussed.
定期监测蚊虫媒介种群是大多数病媒控制项目不可或缺的组成部分。有关蚊虫种类组成、感染状况和杀虫剂抗性的当代数据是有效干预的先决条件。为此，我们在创新病媒控制联盟（IVCC）的资助下，基于单一的“封闭管”平台开发了一套高通量检测方法，这些方法共同构成了“病媒种群监测工具”（VPMT）。VPMT可用于筛查蚊虫疾病媒介种群的多种特征，包括冈比亚按蚊复合组和嗜人按蚊的种类鉴定、疟原虫感染检测以及杀虫剂抗性机制鉴定。在本文中，我们重点介绍了构成VPMT的按蚊特异性检测方法，并详细介绍了一种新的狄氏剂抗性Rdl检测方法。讨论了这些工具的应用、基于非洲实地测试的使用通用和具体指南以及进一步开发计划。
