Institute of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan, Republic of China.
Appl Spectrosc. 2010 Jul;64(7):708-13. doi: 10.1366/000370210791666336.
Recent studies have demonstrated that the autofluorescence technique is a very promising tool for early-stage cancer diagnosis in various tissues. Many researchers have applied the autofluorescence technique through fiber-optic cables to excite tissues and collect the fluorescent emission signal from the tissues for discrimination analysis. In this study, we developed a Monte Carlo-light-induced autofluorescence (MCS LIAF) simulation model to optimize the oblique angle in the excitation optical fiber and the spatial arrangements in the receiving optical fiber. Our aim was to discriminate cervical tissues at different dysplastic stages. The model combined the structure of multi-layered tissues, tissue optical scattering and absorption parameters, tissue fluorophore concentration, the characteristic fluorescent spectrum of fluorophores, and the excitation and receiving arrangement of the optical fibers. The results show that the optimal oblique angle of the excitation optical fiber is between 0 degrees and 45 degrees and that the optimal receiving optical fiber is positioned 200 microm away from the origin. We also observed that changing the excitation angle is very useful in differentiating normal from cervical intraepithelial neoplasia (CIN) I or CIN II tissues. Also, using the fluorescence peak ratio of NADH/collagen can help discriminate CIN III from normal tissues and CIN I/II tissues.
最近的研究表明，自发荧光技术是一种很有前途的工具，可用于各种组织的早期癌症诊断。许多研究人员已经通过光纤电缆应用自发荧光技术来激发组织并从组织中收集荧光发射信号，以进行判别分析。在这项研究中，我们开发了一个蒙特卡罗光诱导自发荧光（MCS LIAF）模拟模型，以优化激发光纤中的倾斜角和接收光纤中的空间排列。我们的目的是区分不同发育阶段的宫颈组织。该模型结合了多层组织的结构、组织光学散射和吸收参数、组织荧光团浓度、荧光团的特征荧光光谱以及光纤的激发和接收排列。结果表明，激发光纤的最佳倾斜角在 0 度到 45 度之间，最佳接收光纤位于距原点 200 微米处。我们还观察到，改变激发角度对于区分正常和宫颈上皮内瘤变（CIN）I 或 CIN II 组织非常有用。此外，使用 NADH/胶原的荧光峰比可以帮助区分 CIN III 与正常组织和 CIN I/II 组织。
