荧光光谱法：一种用于宫颈上皮内瘤变（CIN）的诊断工具。

Fluorescence spectroscopy: a diagnostic tool for cervical intraepithelial neoplasia (CIN).

作者信息

Ramanujam N, Mitchell M F, Mahadevan A, Thomsen S, Silva E, Richards-Kortum R

机构信息

Biomedical Engineering Program, University of Texas, Austin 78712.

出版信息

Gynecol Oncol. 1994 Jan;52(1):31-8. doi: 10.1006/gyno.1994.1007.

DOI:10.1006/gyno.1994.1007

PMID:8307499

Abstract

A spectroscopic system incorporating a pulsed nitrogen laser, an optical fiber probe, and an optical multichannel analyzer was utilized to record fluorescence spectra of the intact cervix at colposcopy. Spectra were obtained from 66 colposcopically normal areas and 49 histologically abnormal areas (5 pathologic inflammation, 21 HPV infection, 9 CIN I, 10 CIN II, and 4 CIN III) in 28 patients. The resulting spectra could be used to differentiate histologically abnormal tissues from colposcopically normal tissues with a sensitivity, specificity, and positive predictive value of 92, 90, and 88%. Furthermore, CIN could be differentiated from nonneoplastic abnormal tissues with a sensitivity, specificity, and positive predictive value of 87, 73, and 74%. These results suggest that laser-induced fluorescence can be used in the recognition and differential diagnosis of CIN at colposcopy.

摘要

一个包含脉冲氮激光器、光纤探头和光学多通道分析仪的光谱系统被用于在阴道镜检查时记录完整宫颈的荧光光谱。从28名患者的66个阴道镜检查正常区域和49个组织学异常区域（5例病理性炎症、21例HPV感染、9例CIN I、10例CIN II和4例CIN III）获取了光谱。所得光谱可用于区分组织学异常组织和阴道镜检查正常组织，其敏感性、特异性和阳性预测值分别为92%、90%和88%。此外，CIN可与非肿瘤性异常组织区分开来，其敏感性、特异性和阳性预测值分别为87%、73%和74%。这些结果表明，激光诱导荧光可用于阴道镜检查时CIN的识别和鉴别诊断。