Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
Antiviral Res. 2010 Oct;88(1):10-8. doi: 10.1016/j.antiviral.2010.06.008. Epub 2010 Jul 6.
Strictinin, which is a member of the ellagitanin family of hydrolyzable tannins, prevented replication of human, duck and swine influenza A viruses (IAVs) in vitro at non-toxic concentrations. The addition of strictinin at the same time as IAV inoculation to MDCK cells inhibited viral replication in a dose-dependent manner. Strictinin showed 50% inhibitory concentrations for IAVs from 0.09±0.021 to 0.28±0.037μM (mean±S.E.M.) by the focus-forming assay. Treatment of MDCK cells with strictinin before and after viral inoculation resulted in no significant antiviral activity. Further studies showed that strictinin inhibited IAV-induced hemifusion. However, strictinin exhibited no inhibitory effect against receptor binding, sialidase activity. Strictinin also showed an antiviral effect on influenza B virus and human parainfluenza virus type-1 in vitro. The results indicate that strictinin is a useful antiviral agent.
鞣花单宁是可水解单宁中的一员，它可以在非毒性浓度下抑制甲型流感病毒（IAV）在体外的复制。鞣花单宁在接种 IAV 的同时添加到 MDCK 细胞中，以剂量依赖性方式抑制病毒复制。鞣花单宁通过空斑形成测定法显示出对 0.09±0.021 至 0.28±0.037μM（平均值±S.E.M.）IAV 的 50%抑制浓度。在病毒接种前后用鞣花单宁处理 MDCK 细胞不会导致明显的抗病毒活性。进一步的研究表明，鞣花单宁抑制 IAV 诱导的半融合。然而，鞣花单宁对受体结合、唾液酸酶活性没有抑制作用。鞣花单宁在体外还对乙型流感病毒和人类副流感病毒 1 型显示出抗病毒作用。结果表明，鞣花单宁是一种有用的抗病毒药物。
