Screening of clones containing altered DNA conformations by plasmid topoprofile shift.
作者信息
Ramesh N, Mishra R K
机构信息
Molecular Biophysics Unit, Indian Institute of Science, Bangalore.
出版信息
J Biochem Biophys Methods. 1991 Feb-Mar;22(2):171-5. doi: 10.1016/0165-022x(91)90030-z.
PMID:2061564
Abstract
DNA topoprofile of supercoiled plasmid is influenced by the presence of altered structures in some stretches. We describe a non-radioactive method for screening the clones containing such altered structures.
摘要
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