• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
DNA curvature influences the internal motions of supercoiled DNA.DNA弯曲影响超螺旋DNA的内部运动。
EMBO J. 1993 Nov;12(11):4407-12. doi: 10.1002/j.1460-2075.1993.tb06125.x.
2
Osmium tetroxide probing of local DNA structure in linear and supercoiled plasmids containing curvature-inducing sequences.四氧化锇对含有曲率诱导序列的线性和超螺旋质粒中局部DNA结构的探测。
Gen Physiol Biophys. 1988 Aug;7(4):379-93.
3
Salt effects on internal motions of superhelical and linear pUC8 DNA. Dynamic light scattering studies.盐对超螺旋和线性pUC8 DNA内部运动的影响。动态光散射研究。
Biophys Chem. 1987 Sep;27(3):263-71. doi: 10.1016/0301-4622(87)80066-2.
4
Action at a distance in supercoiled DNA: effects of sequence on slither, branching, and intramolecular concentration.超螺旋DNA中的远程作用：序列对滑动、分支和分子内浓度的影响。
Biophys J. 1996 Apr;70(4):1893-908. doi: 10.1016/S0006-3495(96)79754-0.
5
Configurational and dynamic properties of different length superhelical DNAs measured by dynamic light scattering.通过动态光散射测量不同长度超螺旋DNA的构型和动力学性质。
Biophys Chem. 1989 Sep 15;34(1):9-18. doi: 10.1016/0301-4622(89)80036-5.
6
Supercoiling-induced DNA bending.超螺旋诱导的DNA弯曲。
Biochemistry. 2004 Aug 24;43(33):10664-8. doi: 10.1021/bi0362572.
7
DNA secondary structure and Raman markers of supercoiling in Escherichia coli plasmid pUC19.大肠杆菌质粒pUC19中DNA二级结构与超螺旋的拉曼标记
Biochemistry. 2002 Jan 22;41(3):847-53. doi: 10.1021/bi011004z.
8
The effect of the DNA conformation on the rate of NtrC activated transcription of Escherichia coli RNA polymerase.sigma(54) holoenzyme.DNA构象对大肠杆菌RNA聚合酶σ⁵⁴全酶NtrC激活转录速率的影响。
J Mol Biol. 2000 Jul 21;300(4):709-25. doi: 10.1006/jmbi.2000.3921.
9
Competing B-Z and helix-coil conformational transitions in supercoiled plasmid DNA.超螺旋质粒DNA中相互竞争的B-Z和螺旋-线圈构象转变
J Biol Chem. 1992 Jan 25;267(3):1776-85.
10
Modulation of intramolecular interactions in superhelical DNA by curved sequences: a Monte Carlo simulation study.弯曲序列对超螺旋DNA分子内相互作用的调控：蒙特卡罗模拟研究
Biophys J. 1995 Jan;68(1):81-8. doi: 10.1016/S0006-3495(95)80161-X.

引用本文的文献

1
DNA sequence encodes the position of DNA supercoils.DNA 序列编码 DNA 超螺旋的位置。
Elife. 2018 Dec 7;7:e36557. doi: 10.7554/eLife.36557.
2
Internal dynamics of supercoiled DNA molecules.超螺旋DNA分子的内部动力学
Biophys J. 2009 Jun 17;96(12):4951-5. doi: 10.1016/j.bpj.2009.03.056.
3
Salt-dependent DNA superhelix diameter studied by small angle neutron scattering measurements and Monte Carlo simulations.通过小角中子散射测量和蒙特卡罗模拟研究盐依赖性DNA超螺旋直径。
Biophys J. 1998 Dec;75(6):3057-63. doi: 10.1016/S0006-3495(98)77746-X.
4
Looping dynamics of linear DNA molecules and the effect of DNA curvature: a study by Brownian dynamics simulation.线性DNA分子的环状动力学及DNA曲率的影响：布朗动力学模拟研究
Biophys J. 1998 Feb;74(2 Pt 1):773-9. doi: 10.1016/S0006-3495(98)74002-0.
5
On the origin of the temperature dependence of the supercoiling free energy.关于超螺旋自由能的温度依赖性的起源
Biophys J. 1997 Nov;73(5):2688-701. doi: 10.1016/S0006-3495(97)78297-3.
6
Salt effects on the structure and internal dynamics of superhelical DNAs studied by light scattering and Brownian dynamics.通过光散射和布朗动力学研究盐对超螺旋DNA结构和内部动力学的影响。
Biophys J. 1997 Nov;73(5):2674-87. doi: 10.1016/S0006-3495(97)78296-1.
7
Brownian dynamics simulations of supercoiled DNA with bent sequences.具有弯曲序列的超螺旋DNA的布朗动力学模拟
Biophys J. 1996 Aug;71(2):955-71. doi: 10.1016/S0006-3495(96)79299-8.
8
Action at a distance in supercoiled DNA: effects of sequence on slither, branching, and intramolecular concentration.超螺旋DNA中的远程作用：序列对滑动、分支和分子内浓度的影响。
Biophys J. 1996 Apr;70(4):1893-908. doi: 10.1016/S0006-3495(96)79754-0.
9
Effect of supercoiling on the juxtaposition and relative orientation of DNA sites.超螺旋对DNA位点的并列及相对取向的影响。
Biophys J. 1996 Jun;70(6):2548-56. doi: 10.1016/S0006-3495(96)79826-0.
10
Sequence-specific labeling of superhelical DNA by triple helix formation and psoralen crosslinking.通过三链螺旋形成和补骨脂素交联对超螺旋DNA进行序列特异性标记。
Nucleic Acids Res. 1996 May 1;24(9):1702-9. doi: 10.1093/nar/24.9.1702.

本文引用的文献

1
The Escherichia coli FIS protein is not required for the activation of tyrT transcription on entry into exponential growth.在进入指数生长期时，激活tyrT转录不需要大肠杆菌FIS蛋白。
EMBO J. 1993 Jun;12(6):2483-94. doi: 10.1002/j.1460-2075.1993.tb05903.x.
2
Torison dynamics and depolarization of fluorescence of linear macromolecules. II. Fluorescence polarization anisotropy measurements on a clean viral phi 29 DNA.线性大分子的扭转动力学与荧光去极化。II. 清洁的φ29病毒DNA的荧光偏振各向异性测量
Biophys Chem. 1980 Oct;12(2):177-88. doi: 10.1016/0301-4622(80)80050-0.
3
Flipping of cloned d(pCpG)n.d(pCpG)n DNA sequences from right- to left-handed helical structure by salt, Co(III), or negative supercoiling.通过盐、钴（III）或负超螺旋使克隆的d(pCpG)n.d(pCpG)n DNA序列从右手螺旋结构转变为左手螺旋结构。
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4560-4. doi: 10.1073/pnas.79.15.4560.
4
Torsional rigidity of DNA and length dependence of the free energy of DNA supercoiling.DNA的扭转刚性与DNA超螺旋自由能的长度依赖性
J Mol Biol. 1984 Feb 15;173(1):75-91. doi: 10.1016/0022-2836(84)90404-2.
5
Anomalous electrophoresis of deoxyribonucleic acid restriction fragments on polyacrylamide gels.脱氧核糖核酸限制片段在聚丙烯酰胺凝胶上的异常电泳。
Biochemistry. 1983 Dec 20;22(26):6186-93. doi: 10.1021/bi00295a023.
6
DNA topoisomerases.DNA拓扑异构酶
Sci Am. 1982 Jul;247(1):94-7, 100-9. doi: 10.1038/scientificamerican0782-94.
7
The facile generation of covalently closed, circular DNAs with defined negative superhelical densities.以确定的负超螺旋密度轻松生成共价闭合的环状DNA。
Anal Biochem. 1982 May 15;122(2):253-7. doi: 10.1016/0003-2697(82)90277-9.
8
The inverted repeat as a recognizable structural feature in supercoiled DNA molecules.反向重复序列作为超螺旋DNA分子中一种可识别的结构特征。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6468-72. doi: 10.1073/pnas.77.11.6468.
9
Sequence specificity of curved DNA.弯曲DNA的序列特异性。
FEBS Lett. 1986 Jan 20;195(1-2):53-6. doi: 10.1016/0014-5793(86)80128-4.
10
Salt effects on internal motions of superhelical and linear pUC8 DNA. Dynamic light scattering studies.盐对超螺旋和线性pUC8 DNA内部运动的影响。动态光散射研究。
Biophys Chem. 1987 Sep;27(3):263-71. doi: 10.1016/0301-4622(87)80066-2.

DNA弯曲影响超螺旋DNA的内部运动。

DNA curvature influences the internal motions of supercoiled DNA.

作者信息

Kremer W, Klenin K, Diekmann S, Langowski J

机构信息

EMBL, Grenoble Outstation, France.

出版信息

EMBO J. 1993 Nov;12(11):4407-12. doi: 10.1002/j.1460-2075.1993.tb06125.x.

DOI:10.1002/j.1460-2075.1993.tb06125.x
PMID:8223450
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC413738/
Abstract

We present evidence that short curved DNA segments can act as mediators for the ordering of large domains in superhelical DNA. Using a non-invasive solution method (dynamic light scattering), we investigated the effect of permanently curved inserts on the solution structure and on the internal motions of superhelical plasmid DNA. We find that the dynamics of superhelical DNA are strongly influenced by sequence- or protein-induced bending: in superhelical plasmids containing curved inserts the amplitude of the internal motion is lower than that of non-curved controls. Furthermore, the relative arrangement of curved sequences in the plasmids can influence the overall shape of the superhelical DNA. On linearized forms of the plasmids, these effects are not observed.

摘要

我们提供的证据表明，短的弯曲DNA片段可以作为超螺旋DNA中大片段有序排列的介质。使用一种非侵入性的溶液方法（动态光散射），我们研究了永久性弯曲插入片段对溶液结构以及超螺旋质粒DNA内部运动的影响。我们发现，超螺旋DNA的动力学受到序列或蛋白质诱导弯曲的强烈影响：在含有弯曲插入片段的超螺旋质粒中，内部运动的幅度低于非弯曲对照。此外，质粒中弯曲序列的相对排列会影响超螺旋DNA的整体形状。在质粒的线性化形式上，未观察到这些效应。