[通过化学结合固定于纤维素衍生物上的脲酶的性质]
[Properties of urease immobilized by chemical binding to a derivatives of cellulose].
作者信息
Fesenko R I, Artemova Iu V, Malakhov A A, Virnik A D, Iakovlev V A
出版信息
Prikl Biokhim Mikrobiol. 1977 Jul-Aug;13(4):572-6.
PMID:20617
Abstract
A comparative study of properties of soluble urease and urease immobilized by its binding to cellulose derivatives was carried out. Upon chemical binding of urease to cellulose derivatives the Mikhaelis constant and pH optimum for the enzyme action changed insignificantly. Maximum enzymic activity of soluble and immobilized urease occurred at 50 degrees C. The energy of activation of urea hydrolysis by urease during enzyme immobilization also changed insignificantly.
摘要
对可溶性脲酶以及通过与纤维素衍生物结合而固定化的脲酶的性质进行了比较研究。脲酶与纤维素衍生物发生化学结合后，酶作用的米氏常数和最适pH值变化不明显。可溶性脲酶和固定化脲酶的最大酶活性均出现在50摄氏度。酶固定化过程中脲酶催化尿素水解的活化能变化也不明显。
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