[微胶囊中脲酶的活性]

[Activity of urease in microcapsules].

作者信息

Ristau O, Pommerening K, Jung C, Rein H, Scheler W

出版信息

Biomed Biochim Acta. 1985;44(7-8):1105-11.

Abstract

The activity of urease in microcapsules formed by a symplex membrane from cellulose sulphate and polydimethyldiallyl ammonium chloride is studied and compared with that of free urease in solution. Both free and encapsulated urease exhibits a similar pH optimum. The interaction of urease with cellulose sulphate causes a decrease of the activity down to 35 to 38%. In spite of its lower absolute activity the encapsulated urease shows a higher long time stability than the free urease. The presented immobilization method for urease could be of importance for an active detoxification in uremia.

摘要

研究了由硫酸纤维素和聚二甲基二烯丙基氯化铵形成的复合膜微胶囊中脲酶的活性，并与溶液中游离脲酶的活性进行了比较。游离脲酶和包封脲酶都表现出相似的最适pH值。脲酶与硫酸纤维素的相互作用导致活性降低至35%至38%。尽管包封脲酶的绝对活性较低，但它比游离脲酶具有更高的长期稳定性。所提出的脲酶固定化方法对于尿毒症的活性解毒可能具有重要意义。