Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Microbiol Immunol. 2010 Jul;54(7):398-404. doi: 10.1111/j.1348-0421.2010.00233.x.
Campylobacter fetus is divided into CFV and CFF. Because CFV causes bovine genital campylobacteriosis, differentiation of the two subspecies is essential to the implementation of efficient CFV control and eradication programs. We have developed LAMP and duplex PCR assays for rapid and simple detection of CFV. The LAMP assay correctly detected 7 CFV strains and did not detect 53 CFF, 35 non-fetus Campylobacter and 25 non-Campylobacter strains. The PCR assay successfully differentiated the two subspecies. The LAMP and PCR assays were faster than conventional biochemical assays, requiring for detection less than 50 min and less than 4 hr, respectively, from the beginning of DNA extraction from a single colony on blood agar to final determination. Our LAMP and PCR assays are rapid and practical tools for detection of CFV.
胎儿弯曲菌分为 CFV 和 CFF。由于 CFV 引起牛生殖道弯曲菌病，因此区分这两个亚种对于实施有效的 CFV 控制和根除计划至关重要。我们已经开发了 LAMP 和双重 PCR 检测方法，用于快速简单地检测 CFV。LAMP 检测法正确检测了 7 株 CFV 菌株，未检测到 53 株 CFF、35 株非胎儿弯曲菌和 25 株非弯曲菌菌株。PCR 检测法成功地区分了这两个亚种。LAMP 和 PCR 检测法比传统的生化检测法更快，从单个血琼脂平板上的单个菌落提取 DNA 开始，分别需要不到 50 分钟和不到 4 小时即可完成检测。我们的 LAMP 和 PCR 检测法是快速实用的 CFV 检测工具。
