Tonutti Laura, Manzi Luca, Tacconi Maria T, Bazzoni Gianfranco
Mario Negri Institute of Pharmacological Research, Via La Masa 19, Milano, Italy.
J Angiogenes Res. 2010 Jul 9;2:12. doi: 10.1186/2040-2384-2-12.
As n-3 Polyunsaturated Fatty Acids exert a beneficial action on the cardiovascular system, it is important to investigate their effects on endothelial cell responses that (like migration) contribute to repairing vascular lesions.
To this purpose, using functional and morphological in vitro assays, we have examined the effect of n-3 Polyunsaturated Fatty Acids on the migration of endothelial cells.
We report here that incubation of endothelial cells with n-3 Polyunsaturated Fatty Acids impaired cell migration into a wound, triggered peripheral distribution of focal adhesions and caused partial disassembly of actin filaments. We also found that eicosapentaenoic acid and docosahexaenoic acid exerted similar effects on the focal adhesions, but that eicosapentaenoic acid was sufficient for inhibiting cell migration.
Given the importance of endothelial cell migration in the repair of vascular injuries, these in vitro findings call for in vivo evaluation of vascular repair in response to different dietary ratios of eicosapentaenoic to docosahexaenoic acid.
由于n-3多不饱和脂肪酸对心血管系统具有有益作用，因此研究它们对内皮细胞反应（如迁移）的影响非常重要，这些反应有助于修复血管损伤。
为此，我们使用功能和形态学体外试验，研究了n-3多不饱和脂肪酸对内皮细胞迁移的影响。
我们在此报告，用n-3多不饱和脂肪酸孵育内皮细胞会损害细胞向伤口的迁移，引发粘着斑的外周分布，并导致肌动蛋白丝的部分解聚。我们还发现，二十碳五烯酸和二十二碳六烯酸对粘着斑有类似影响，但二十碳五烯酸足以抑制细胞迁移。
鉴于内皮细胞迁移在血管损伤修复中的重要性，这些体外研究结果要求对不同饮食比例的二十碳五烯酸与二十二碳六烯酸所引起的血管修复进行体内评估。
