Ouyang Hua-Qiang, Liu Lu-Ming, Chen Zhen, Luo Jian-Min, Yu Er-Xin
Department of Integrated Traditional Chinese and Western Medicine, Cancer Hospital, Fudan University, Shanghai 200032, China.
Zhong Xi Yi Jie He Xue Bao. 2010 Jul;8(7):655-61. doi: 10.3736/jcim20100709.
To investigate the effects of Qingyi Huaji (QYHJ) decoction, a compound traditional Chinese herbal medicine, on tumor inhibition rate and serum levels of interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-alpha) in nude mice with transplanted tumors of human pancreatic cancer.
The tumor-bearing mice model was established by subcutaneously inoculating with xenografts of pancreatic cancer into the right armpit of 40 BALB/c nude mice. After successful modeling, the mice were randomly divided into untreated group (Arabic gum), capecitabine group, low-dose QYHJ decoction group (36 g/kg) and high-dose QYHJ decoction group (72 g/kg), with 10 mice in each group. Citrate buffer solution (containing 5% Arabic gum), capecitabine suspension and QYHJ decoction were administered to four groups by gavage respectively. After 5-week treatment, the concentrations of serum IL-6, IL-8 and TNF-alpha were examined by enzyme-linked immunosorbent assay (ELISA) using blood sample from eye socket. Then the mice were euthanized by cervical dislocation. Tumor weight and the tumor inhibition rate were calculated.
Tumor weight in the low-dose QYHJ decoction group decreased significantly as compared with the untreated group (P<0.05). Serum levels of IL-6 and TNF-alpha in low- and high-dose QYHJ groups were extremely significantly lower than those in the untreated group (P<0.01). Serum level of IL-8 in the low-dose QYHJ group was significantly lower than that in the untreated group (P<0.05). Correlation analysis showed that transplanted tumor weight of the mice was linearly positively correlated with serum levels of IL-6, IL-8 or TNF-alpha (P<0.01).
Conventional dose of QYHJ decoction is effective in suppressing pancreatic carcinoma in nude mice. The mechanism may be related to down-regulation of serum cytokines such as IL-6, IL-8 and TNF-alpha.
研究复方中药清胰化积（QYHJ）汤对人胰腺癌移植瘤裸鼠的抑瘤率及血清白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）水平的影响。
将40只BALB/c裸鼠右腋皮下接种人胰腺癌异种移植瘤，建立荷瘤小鼠模型。造模成功后，将小鼠随机分为空白对照组（阿拉伯胶）、卡培他滨组、QYHJ汤低剂量组（36 g/kg）和QYHJ汤高剂量组（72 g/kg），每组10只。分别对四组小鼠灌胃给予枸橼酸盐缓冲溶液（含5%阿拉伯胶）、卡培他滨混悬液和QYHJ汤。治疗5周后，采集眼眶血，采用酶联免疫吸附测定（ELISA）法检测血清IL-6、IL-8和TNF-α浓度。然后颈椎脱臼处死小鼠，计算肿瘤重量和抑瘤率。
QYHJ汤低剂量组肿瘤重量较空白对照组明显减轻（P<0.05）。QYHJ汤低、高剂量组血清IL-6和TNF-α水平极显著低于空白对照组（P<0.01）。QYHJ汤低剂量组血清IL-8水平显著低于空白对照组（P<0.05）。相关性分析显示，小鼠移植瘤重量与血清IL-6、IL-8或TNF-α水平呈线性正相关（P<0.01）。
常规剂量的QYHJ汤对裸鼠胰腺癌具有抑制作用。其机制可能与下调血清IL-6、IL-8和TNF-α等细胞因子有关。
