Wang Peng, Liu Lu-Ming, Chen Zhen
Department of Integrative Oncology, Fudan University Shanghai Cancer Center, Shanghai.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 Sep;30(9):942-5.
To explore the action mechanism of action of Qingyi Huaji Formula (QYHJ), whether its inhibition on the growth of the tumor is by way of down-regulating Ski expression.
SW1990 tumor cell with low Ski expression was created by lenti-virus mediated RNA interfering technique. Tumor cells with different levels of Ski expression were subcutaneously transplanted to nude mice, and the response of cancer cells to QYHJ treatment was detected.
After QYHJ treatment, the tumor growth slowed-down significantly, with the Ski mRNA and protein expressions in tumor reduced by 39.6% and 41.3% of that in untreated tumor respectively (P < 0.05). Ski mRNA and protein expressions in the created high and low Ski expression tumor cells were 105%, 123% and 46%, 30% respectively of that in parental cells (P < 0.05). The tumor weight inhibitory rates of QYHJ on high Ski expression cells were 29.6% and 32.2%, while on low Ski expression cells, it was 16.0% to 17.8% (P < 0.05).
Ski acts as an important therapeutic target of QYHJ in treating SW1990 pancreatic cancer, and its expression status mediates different response of tumor cells to QYHJ treatment.
探讨清胰化积方（QYHJ）的作用机制，其对肿瘤生长的抑制是否通过下调Ski表达来实现。
采用慢病毒介导的RNA干扰技术构建Ski表达低的SW1990肿瘤细胞。将不同Ski表达水平的肿瘤细胞皮下移植到裸鼠体内，检测癌细胞对QYHJ治疗的反应。
QYHJ治疗后，肿瘤生长明显减缓，肿瘤中Ski mRNA和蛋白表达分别比未治疗肿瘤降低39.6%和41.3%（P<0.05）。构建的高、低Ski表达肿瘤细胞中Ski mRNA和蛋白表达分别为亲代细胞的105%、123%和46%、30%（P<0.05）。QYHJ对高Ski表达细胞的肿瘤重量抑制率为29.6%和32.2%，对低Ski表达细胞的抑制率为16.0%至17.8%（P<0.05）。
Ski是QYHJ治疗SW1990胰腺癌的重要治疗靶点，其表达状态介导肿瘤细胞对QYHJ治疗的不同反应。
