Boyd J, Pienta K J, Getzenberg R H, Coffey D S, Barrett J C
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
J Natl Cancer Inst. 1991 Jun 19;83(12):862-6. doi: 10.1093/jnci/83.12.862.
Alterations of nuclear shape are frequently observed in tumor cells, but the genes controlling these changes and the stage in the neoplastic process at which they occur are unknown. We have studied nuclear shape changes in chemically immortalized, nontumorigenic Syrian hamster embryo cell clones that had either retained (supB+) or lost (supB-) the ability to suppress the tumorigenic phenotype when they were hybridized with a tumor cell line (BP6T). Quantitative morphometric analysis of the nuclei of cells from each of two pairs of supB+/supB- variants indicated that the nuclei of supB- cells were significantly more out of round than those of their corresponding supB+ clones. These data indicate that modification of nuclear structure may represent an early, preneoplastic event in multistep chemical carcinogenesis and that loss of a tumor suppressor gene function may regulate alterations in nuclear morphology.
肿瘤细胞中经常观察到核形态的改变，但控制这些变化的基因以及它们在肿瘤形成过程中发生的阶段尚不清楚。我们研究了化学永生化的、无致瘤性的叙利亚仓鼠胚胎细胞克隆中的核形态变化，这些克隆在与肿瘤细胞系（BP6T）杂交时，要么保留了（supB+），要么失去了（supB-）抑制致瘤表型的能力。对两对supB+/supB-变体中每个细胞的细胞核进行定量形态计量分析表明，supB-细胞的细胞核比其相应的supB+克隆的细胞核明显更不圆。这些数据表明，核结构的改变可能代表多步化学致癌过程中的早期肿瘤前事件，并且肿瘤抑制基因功能的丧失可能调节核形态的改变。
