构建一株弗氏志贺菌生物发光报告质粒。
Construction of a bioluminescence reporter plasmid for Francisella tularensis.
机构信息
The University of Tennessee Health Science Center, Department of Molecular Sciences, Memphis, TN 38163, USA.
出版信息
Plasmid. 2010 Nov;64(3):156-61. doi: 10.1016/j.plasmid.2010.07.001. Epub 2010 Jul 8.
Abstract
A Francisella tularensis shuttle vector that constitutively expresses the Photorhabdus luminescens lux operon in type A and type B strains of F. tularensis was constructed. The bioluminescence reporter plasmid was introduced into the live vaccine strain of F. tularensis and used to follow F. tularensis growth in a murine intranasal challenge model in real-time by bioluminescence imaging. The results show that the new bioluminescence reporter plasmid represents a useful tool for tularemia research that is suitable for following F. tularensis growth in both in vitro and in vivo model systems.
摘要
构建了一种弗朗西斯氏土拉菌穿梭载体，可在弗朗西斯氏土拉菌的 A 型和 B 型菌株中组成型表达 Photorhabdus luminescens lux 操纵子。生物发光报告质粒被引入弗朗西斯氏土拉菌活疫苗株中，并通过生物发光成像实时跟踪在小鼠鼻腔内挑战模型中的弗朗西斯氏土拉菌生长。结果表明，新的生物发光报告质粒代表了一种用于土拉菌病研究的有用工具，适用于在体外和体内模型系统中跟踪弗朗西斯氏土拉菌的生长。
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