Laboratory of Animal Breeding and Genetics, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan,
Biochem Genet. 2010 Oct;48(9-10):829-39. doi: 10.1007/s10528-010-9364-x. Epub 2010 Jul 11.
Six polymorphic sites of the bovine prion protein gene (PRNP) were genotyped in 569 animals of Asian native cattle, Japanese breeds, purebred mythun (Bos frontalis), and mythun x cattle composite animals. At the 23-bp indel site, a deletion (23-) allele was a major allele in all populations except mythun. At the 12-bp indel site, an insertion (12+) allele was a major allele in all populations. The 14-bp indel site was polymorphic in all Asian native cattle. In the octapeptide repeat region, a six-repeat allele was a major allele in all populations, and 5/5 and 4/6 genotypes were detected in Japanese Black and Mongolian cattle and in mythun, respectively. Two nonsynonymous single nucleotide polymorphisms (SNPs) (K3T and S154N) were detected in Asian native cattle and mythun. Haplotype analysis using the genotypes of the six sites estimated 33 different haplotypes. The haplotype 23- 12- K 6 S 14+ was found in all populations.
对 569 头亚洲本地牛、日本品种牛、纯种野牛（Bos frontalis）和野牛与牛的杂交后代进行了牛朊蛋白基因（PRNP）的 6 个多态性位点的基因型分析。在 23-bp 插入缺失位点，除了野牛之外，所有群体均以缺失（23-）等位基因为主要等位基因。在 12-bp 插入缺失位点，所有群体均以插入（12+）等位基因为主要等位基因。在所有亚洲本地牛中，14-bp 插入缺失位点呈多态性。在八肽重复区，所有群体均以六重复等位基因为主要等位基因，在日本黑牛和蒙古牛以及野牛中分别检测到 5/5 和 4/6 基因型。在亚洲本地牛和野牛中检测到两个非同义单核苷酸多态性（SNPs）（K3T 和 S154N）。利用六个位点的基因型进行单倍型分析，估计了 33 种不同的单倍型。所有群体均存在 23- 12- K 6 S 14+ 单倍型。
