Kyburz Annika, Raulinaitis Vytautas, Koskela Outi, Kontinen Vesa, Permi Perttu, Kilpelainen Ilkka, Seppala Raili
Laboratory of Organic Chemistry, Department of Chemistry, University of Helsinki, A.I. Virtasen Aukio 1, P.O. Box 55, 00014, Helsinki, Finland.
Biomol NMR Assign. 2010 Oct;4(2):235-8. doi: 10.1007/s12104-010-9249-2. Epub 2010 Jul 11.
MreB, MreC and MreD are essential cell shape-determining morphogenetic proteins in Gram-positive and in Gram-negative bacteria. While MreB, the bacterial homologue of the eukaryotic cytoskeletal protein actin, has been extensively studied, the roles of MreC and MreD are less well understood. They both are transmembrane proteins. MreC has a predicted single transmembrane domain and the C-terminal part outside the cell membrane. MreC probably functions as a link between the intracellular cytoskeleton and the cell wall synthesizing machinery which is located at the outer surface of the cell membrane. Also proteins involved in cell wall synthesis participate in cell morphogenesis. How these two processes are coordinated is, however, poorly understood. Bacillus subtilis (BS), a non-pathogenic Gram-positive bacterium, is widely used as a model for Gram-positive pathogens, e.g. Staphylococcus aureus (SA). Currently, the structures of MreC from BS and SA are not known. As part of our efforts to elucidate the structure-function relationships of the morphogenetic protein complexes in Gram-positive bacteria, we present the backbone and side chain resonance assignments of the extracytoplasmic domain of MreC from BS.
MreB、MreC和MreD是革兰氏阳性菌和革兰氏阴性菌中决定细胞形状的必需形态发生蛋白。虽然作为真核细胞骨架蛋白肌动蛋白的细菌同源物，MreB已得到广泛研究，但MreC和MreD的作用却了解较少。它们都是跨膜蛋白。MreC有一个预测的单一跨膜结构域，其C端部分位于细胞膜外。MreC可能作为细胞内细胞骨架与位于细胞膜外表面的细胞壁合成机制之间的连接物。参与细胞壁合成的蛋白质也参与细胞形态发生。然而，这两个过程是如何协调的却知之甚少。枯草芽孢杆菌（BS）是一种非致病性革兰氏阳性菌，被广泛用作革兰氏阳性病原体（如金黄色葡萄球菌（SA））的模型。目前，来自BS和SA的MreC的结构尚不清楚。作为我们阐明革兰氏阳性菌中形态发生蛋白复合物结构 - 功能关系工作的一部分，我们给出了来自BS的MreC胞外结构域的主链和侧链共振归属。
