Pereira Ana C, Paiva Ana, Saraiva Ivo H, Costa Teresa, Henriques Adriano O, Matzapetakis Manolis
Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157, Oeiras, Portugal.
Biomol NMR Assign. 2015 Oct;9(2):285-8. doi: 10.1007/s12104-014-9593-8. Epub 2014 Dec 11.
RodZ (also known as YfgA) is a component of the core bacterial morphogenic apparatus. RodZ is a key cell shape determinant in rod-shaped bacteria and it interacts with the actin-like cytoskeletal protein MreB. In Bacillus subtilis, this 304-residue transmembrane protein is composed of three distinct domains: a cytoplasmic domain (RodZn), a transmembrane domain, and an extra-cytoplasmic domain (RodZc). Here we report the (1)H, (13)C and (15)N backbone and side chain resonance assignments of the RodZc domain from B. subtilis by NMR spectroscopy, and the resulting secondary structure prediction.
RodZ（也称为YfgA）是细菌核心形态发生装置的一个组成部分。RodZ是杆状细菌中关键的细胞形状决定因素，它与肌动蛋白样细胞骨架蛋白MreB相互作用。在枯草芽孢杆菌中，这种由304个氨基酸残基组成的跨膜蛋白由三个不同的结构域组成：一个胞质结构域（RodZn）、一个跨膜结构域和一个胞外结构域（RodZc）。在此，我们通过核磁共振光谱法报告了枯草芽孢杆菌RodZc结构域的（1）H、（13）C和（15）N主链及侧链共振归属，以及由此得到的二级结构预测。
