Division of Oral Pathology, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Gakkocho-dori, Chuo-ku, Niigata 951-8514, Japan.
Microsc Res Tech. 2011 Mar;74(3):264-8. doi: 10.1002/jemt.20900.
For histopathological assessment of oral borderline malignancies, it is important to carefully detect subtle epithelial changes on fully stretched tissue sections. However, it is not generally easy to obtain wrinkle-free sections when using formalin-fixed paraffin-embedded oral mucosal samples. Since acetic acid treatment is already utilized for large brain tissue sections, we examined whether that treatment was also effective for oral mucosal tissues containing normal to malignant epithelial lesions. Paraffin sections were floated in various concentrations of acetic acid for 10 min after stretching in water for 1 min, then wrinkle formations were examined using hematoxylin and eosin staining, as well as for staining intensity with keratin immunohistochemistry. Wrinkles were formed in both epithelial and connective tissue zones of sections treated with less than a 40-mM (0.25%) concentration of acetic acid. In contrast, treatments with concentrations at 80 mM (0.5%) and higher resulted in cracking between the epithelial layer and lamina propria, as well as poor immunohistochemical results for keratins 13 and 17, even though the wrinkles completely disappeared. These results indicate that 40 mM is the optimal concentration of acetic acid solution to prevent wrinkles in the epithelial layer while maintaining the immunohistochemical qualities of oral mucosa tissue sections, especially those containing borderline malignant epithelial lesions.
对于口腔交界性恶性肿瘤的组织病理学评估，重要的是要在充分拉伸的组织切片上仔细检测细微的上皮变化。然而，在用福尔马林固定的石蜡包埋口腔黏膜样本获得无皱纹的切片通常并不容易。由于醋酸处理已经用于大型脑组织切片，我们研究了该处理是否也对含有正常至恶性上皮病变的口腔黏膜组织有效。石蜡切片在水中拉伸 1 分钟后，在各种浓度的醋酸中漂浮 10 分钟，然后用苏木精和伊红染色以及角蛋白免疫组织化学染色检查皱纹形成情况。用小于 40mM（0.25%）浓度的醋酸处理的切片的上皮和结缔组织区域均形成皱纹。相比之下，用 80mM（0.5%）及更高浓度的醋酸处理会导致上皮层和固有层之间出现裂缝，以及角蛋白 13 和 17 的免疫组织化学结果不佳，尽管皱纹完全消失。这些结果表明，40mM 是醋酸溶液的最佳浓度，可以防止上皮层出现皱纹，同时保持口腔黏膜组织切片的免疫组织化学特性，特别是那些含有交界性恶性上皮病变的切片。
