Reibel J
J Oral Pathol. 1984 Feb;13(1):75-84. doi: 10.1111/j.1600-0714.1984.tb01403.x.
The distribution of immunoglobulins and fibrinogen in normal and inflamed oral mucosa was examined by direct immunofluorescence using enzyme-treated sections of formalin-fixed, paraffin-embedded tissue. Plasma proteins were found inter- and intracellularly in the surface epithelium in most of the normal and all of the inflamed mucosal specimens. This diffusion of plasma proteins into the surface epithelium was shown to contribute to the appearance of edematous epithelial cells and eosinophilic bodies ("keratin pools"). The results clearly demonstrate that oral epithelium is not to be regarded as a covering membrane isolated from the underlying connective tissue. Furthermore, the study confirms that immunohistochemical studies can be performed on enzyme-pretreated sections of routinely fixed and embedded tissue although it is emphasized that the method is not of universal applicability.
采用酶处理的福尔马林固定、石蜡包埋组织切片，通过直接免疫荧光法检测正常和炎症口腔黏膜中免疫球蛋白和纤维蛋白原的分布。在大多数正常和所有炎症黏膜标本的表面上皮细胞内和细胞间均发现血浆蛋白。血浆蛋白向表面上皮细胞的这种扩散导致出现水肿性上皮细胞和嗜酸性小体（“角蛋白池”）。结果清楚地表明，口腔上皮不应被视为与下方结缔组织隔离的覆盖膜。此外，该研究证实，尽管强调该方法并非普遍适用，但可以对常规固定和包埋组织的酶预处理切片进行免疫组织化学研究。
