Nehlig A, Boyet S, Pereira de Vasconcelos A
INSERM U272, Nancy, France.
Neuroscience. 1991;40(3):871-8. doi: 10.1016/0306-4522(91)90018-j.
An autoradiographic method has been developed for the regional assessment of cerebral tracer levels after the acute intravenous injection of [3-14C]beta-hydroxybutyrate in developing rats. The animals were studied at five postnatal stages, i.e. postnatal day 10 (P10), P14, P17, P21 and P35. Tracer levels were high from P10 to P17, reaching peak values at P14, which were two- to threefold higher than those at P10. At P17, tracer concentrations were about twice as low as at P14. Between P17 and P21, regional 14C concentrations were again reduced by about twofold in all areas studied and decreased further by about 50% after weaning reaching quite low levels by P35. The distribution of 14C inside sections appeared to be rather homogeneous throughout the brain at all stages studied, never exceeding a ratio higher than 2 at any stage studied. These results are in good agreement with previous data on the rate of uptake and utilization of beta-hydroxybutyrate by the immature rat brain.
已开发出一种放射自显影方法，用于在发育中的大鼠急性静脉注射[3-14C]β-羟基丁酸酯后对脑示踪剂水平进行区域评估。在五个出生后阶段对动物进行研究，即出生后第10天（P10）、P14、P17、P21和P35。从P10到P17示踪剂水平较高，在P14达到峰值，比P10时高两到三倍。在P17时，示踪剂浓度约为P14时的一半。在P17和P21之间，所有研究区域的14C浓度再次降低约两倍，断奶后进一步降低约50%，到P35时达到相当低的水平。在所有研究阶段，切片内14C的分布在整个大脑中似乎相当均匀，在任何研究阶段都从未超过2的比率。这些结果与先前关于未成熟大鼠脑对β-羟基丁酸酯摄取和利用速率的数据高度一致。
