Crane S C, Morgan B L
J Nutr. 1983 May;113(5):1063-72. doi: 10.1093/jn/113.5.1063.
The effect of alterations in ketone body availability during the early postnatal period on the in vitro and in vivo utilization of beta-hydroxybutyrate (beta OHB) by developing rat brain was determined. Ketone body availability was prolonged by feeding a high fat diet during pregnancy and lactation and to pups after weaning. Availability was decreased by early weaning pups at 16 days to a low fat liquid diet. In the in vitro studies, oxidation of [14C]beta OHB to CO2 and incorporation into brain lipid was determined at 7, 14, 21, and 35 days of age. In the in vivo studies incorporation of [14C]beta OHB and [3H]glucose into brain lipid was measured. The results indicate that it is possible to alter the brain's utilization of beta OHB for lipid synthesis and energy metabolism during the developmental period. This was particularly evident in the in vivo studies. The in vivo results also suggest a relationship between beta OHB availability in the blood and incorporation into brain lipid. When availability was decreased by early weaning pups to the low fat diet, incorporation of beta OHB into lipid was also decreased. Likewise, when availability was increased, such as was seen in the high fat feeding study, incorporation was increased.
研究了产后早期酮体可利用性的改变对发育中大鼠脑体外和体内β-羟基丁酸酯（β-OHB）利用情况的影响。通过在怀孕和哺乳期给母鼠喂食高脂肪饮食，并在断奶后给幼鼠喂食高脂肪饮食来延长酮体的可利用性。通过在16日龄时将幼鼠提前断奶并喂食低脂液体饮食来降低酮体的可利用性。在体外研究中，测定了7、14、21和35日龄时[14C]β-OHB氧化为CO2以及掺入脑脂质的情况。在体内研究中，测量了[14C]β-OHB和[3H]葡萄糖掺入脑脂质的情况。结果表明，在发育期间改变大脑对β-OHB用于脂质合成和能量代谢的利用是可能的。这在体内研究中尤为明显。体内研究结果还表明血液中β-OHB的可利用性与掺入脑脂质之间存在关联。当通过将幼鼠提前断奶至低脂饮食来降低可利用性时，β-OHB掺入脂质的情况也会减少。同样，当可利用性增加时，如在高脂肪喂养研究中所见，掺入量会增加。
