Department of Stem Cell Disorders, Kansai Medical University, Moriguchi City, Osaka, Japan.
Transpl Immunol. 2010 Oct;24(1):33-9. doi: 10.1016/j.trim.2010.06.014. Epub 2010 Jul 29.
Graft versus host disease (GVHD), rejection, delayed immune reconstitution and infections have been significant hurdles to haploidentical BMT. In order to improve the outcome of the current haploidentical-related BMT, we performed a novel BMT method consisting of the perfusion method (PM) plus intra-bone marrow-bone marrow transplantation (IBM-BMT) in a rabbit model. The percentages of T cells in BMCs harvested by the PM and the conventional aspiration method (AM) were 6% and 14%, respectively (p<0.01). Conversely, the CFU-C counts of BMCs in the PM group were significantly higher than those in the AM group. When the BMCs were transplanted into lethally irradiated offspring rabbits by IBM-BMT, hemopoietic recovery in the PM group was faster than in the AM group. The cumulative incidence of acute GVHD was 25% in the PM group versus 75% in the AM group (p<0.05). In addition, the survival rate was 75% in the PM group versus 33% in the AM group (p<0.05). Thus, the new method is able to provide rapid hemopoiesis, reduce the cumulative incidence of acute GVHD, and achieve a higher survival rate. This novel strategy paves the way for new dimensions in haploidentical BMT.
移植物抗宿主病（GVHD）、排斥反应、延迟的免疫重建和感染一直是半相合 BMT 的重大障碍。为了改善当前半相合相关 BMT 的结果，我们在兔模型中进行了一种新的 BMT 方法，包括灌注法（PM）加骨髓内-骨髓内移植（IBM-BMT）。PM 和传统抽吸法（AM）采集的 BMC 中 T 细胞的百分比分别为 6%和 14%（p<0.01）。相反，PM 组 BMC 的 CFU-C 计数明显高于 AM 组。当通过 IBM-BMT 将 BMC 移植到致死性照射的后代兔子中时，PM 组的造血恢复比 AM 组更快。PM 组急性 GVHD 的累积发生率为 25%，而 AM 组为 75%（p<0.05）。此外，PM 组的存活率为 75%，而 AM 组为 33%（p<0.05）。因此，这种新方法能够提供快速的造血，降低急性 GVHD 的累积发生率，并实现更高的存活率。这种新策略为半相合 BMT 开辟了新的维度。
