The First Department of Internal Medicine, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka, 570-8506, Japan.
Department of Stem Cell Disorders, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka, 570-8506, Japan.
Int J Hematol. 2011 Jun;93(6):822-824. doi: 10.1007/s12185-011-0865-0. Epub 2011 May 20.
We previously described a unique procedure for the collection of bone marrow cells (BMCs) using a perfusion method (PM). In cynomolgus monkeys, this method resulted in lower contamination with T cells (<10%). Here, we performed PM on a poor mobilizer lymphoma patient. We confirmed the safety of the intra-bone marrow injection of saline to collect the BMCs. The collected BMCs showed minimal contamination with T cells (<15%) and red blood cells (RBCs) (<4%) from the peripheral blood. It took a total of only 30 min to collect the BMCs. Moreover, transfusion of RBCs was unnecessary. There were no relevant post-operative side effects except for self-limiting pain at the sites of collection, and the patient was able to walk around the hospital after the operation.
我们之前描述了一种使用灌流法（PM）采集骨髓细胞（BMCs）的独特方法。在食蟹猴中，这种方法导致 T 细胞污染率较低（<10%）。在这里，我们对一位动员不佳的淋巴瘤患者进行了 PM。我们确认了向骨髓内注射生理盐水以采集 BMCs 的安全性。从外周血中收集的 BMCs 显示 T 细胞（<15%）和红细胞（RBCs）（<4%）的污染最小。总共仅需 30 分钟即可收集 BMCs。此外，不需要输血。除了采集部位的自限性疼痛外，没有其他相关的术后副作用，患者在手术后能够在医院周围走动。
