波兰人群样本中15个常染色体STR基因座的遗传变异
Genetic variation of 15 autosomal STR loci in a population sample from Poland.
作者信息
Parys-Proszek Agnieszka, Kupiec Tomasz, Wolańska-Nowak Paulina, Branicki Wojciech
机构信息
Institute of Forensic Research, Section of Forensic Genetics, Westerplatte 9, 31-033 Krakow, Poland.
出版信息
Leg Med (Tokyo). 2010 Sep;12(5):246-8. doi: 10.1016/j.legalmed.2010.05.002. Epub 2010 Jul 10.
Fifteen autosomal STR loci included in AmpFlSTR NGM kit were analyzed in 154 unrelated individuals from Poland. This multiplex kit enables simultaneous amplification of 10 standard STR loci included in AmpFlSTR SGM Plus kit (D3S1358, vWA, D16S539, D2S1338, D8S1179, D19S433, TH01, FGA, D21S11 and D18S51) and five new mini- and midi-STR loci (D10S1248, D22S1045, D2S441, D1S1656 and D12S391). Population study was conducted to evaluate usefulness of the loci (especially the five new microsatellite systems) in forensic genetic identification examinations. All 15 markers were found to be in Hardy-Weinberg equilibrium. The combined probability of match for the 15 studied STR loci was 3.998 x 10(-19). The same parameter calculated for five new microsatellite loci equaled 8.83 x 10(-7). Discrimination power was particularly high in case of D1S1656 (0.975) and D12S391 (0.972) STR loci.
对来自波兰的154名无关个体分析了AmpFlSTR NGM试剂盒中包含的15个常染色体STR基因座。该多重试剂盒能够同时扩增AmpFlSTR SGM Plus试剂盒中包含的10个标准STR基因座（D3S1358、vWA、D16S539、D2S1338、D8S1179、D19S433、TH01、FGA、D21S11和D18S51）以及5个新的微型和中型STR基因座（D10S1248、D22S1045、D2S441、D1S1656和D12S391）。进行群体研究以评估这些基因座（尤其是5个新的微卫星系统）在法医遗传学鉴定检查中的实用性。发现所有15个标记均处于哈迪-温伯格平衡。所研究的15个STR基因座的匹配联合概率为3.998×10⁻¹⁹。为5个新的微卫星基因座计算的相同参数等于8.83×10⁻⁷。在D1S1656（0.975）和D12S391（0.972）STR基因座的情况下，鉴别力特别高。
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