克罗地亚东部人群中五个新的欧洲标准集STR基因座（D10S1248、D22S1045、D2S441、D1S1656、D12S391）的遗传参数
Genetic parameters of five new European Standard Set STR loci (D10S1248, D22S1045, D2S441, D1S1656, D12S391) in the population of eastern Croatia.
作者信息
Curic Goran, Gasic Vedran, Pluzaric Vera, Smiljcic Danijela
机构信息
DNA Laboratory, School of Medicine, J. J. Strossmayer University in Osijek, J. Huttlera 4, Osijek, Croatia.
出版信息
Croat Med J. 2012 Oct;53(5):409-15. doi: 10.3325/cmj.2012.53.409.
AIM
To establish allele frequencies and genetic parameters in eastern Croatia population and to compare them with those in other populations. The second aim was to compare the genetic profiles obtained with different forensic kits amplifying the same genetic markers.
METHODS
Blood samples of 217 unrelated individuals from eastern Croatia were genotyped using AmpFlSTR NGM kit. Allele distribution and other genetic parameters were determined for 15 short tandem repeat (STR) loci, including the 5 loci recently added to the European Standard Set (ESS) of STR loci (D10S1248, D22S1045, D2S441, D1S1656, and D12S391). Ninety-six samples underwent duplicate analysis using AmpFlSTR Identifiler kit.
RESULTS
Power of discrimination was highest for the two new ESS loci, D1S1656 (0.97254) and D12S391 (0.97339). Comparison of allele frequencies for 5 new ESS loci in our sample with previously published population data showed a significant difference from Maghreb population on D2S441 and from American Caucasian population on D1S1656. Comparison of allele frequencies for standard 10 STR loci with all the neighboring populations' data showed a significant difference only from Albanian population (on D2S1338, D18S51, and TH01). Discordant genotypes were observed in 5 (5.2%) samples at a single locus when amplified with both AmpFlSTR NGM and AmpFlSTR Identifiler kit.
CONCLUSION
New ESS STR loci are highly polymorphic and short, and therefore very useful for the analysis of challenging forensic samples. DNA samples purposed for establishing databases should be routinely amplified in duplicate.
目的
确定克罗地亚东部人群的等位基因频率和遗传参数，并与其他人群进行比较。第二个目的是比较使用不同法医试剂盒扩增相同遗传标记所获得的基因图谱。
方法
使用AmpFlSTR NGM试剂盒对来自克罗地亚东部的217名无关个体的血液样本进行基因分型。确定了15个短串联重复序列（STR）位点的等位基因分布和其他遗传参数，包括最近添加到欧洲STR位点标准集（ESS）中的5个位点（D10S1248、D22S1045、D2S441、D1S1656和D12S391）。96个样本使用AmpFlSTR Identifiler试剂盒进行了重复分析。
结果
两个新的ESS位点D1S1656（0.97254）和D12S391（0.97339）的鉴别力最高。将我们样本中5个新的ESS位点的等位基因频率与先前公布的人群数据进行比较，结果显示在D2S441位点上与马格里布人群存在显著差异，在D1S1656位点上与美国白种人群存在显著差异。将标准的10个STR位点的等位基因频率与所有相邻人群的数据进行比较，结果显示仅在D2S1338、D18S51和TH01位点上与阿尔巴尼亚人群存在显著差异。当使用AmpFlSTR NGM和AmpFlSTR Identifiler试剂盒扩增时，在5个（5.2%）样本的单个位点上观察到不一致的基因型。
结论
新的ESS STR位点高度多态且短小，因此对于分析具有挑战性法医样本非常有用。用于建立数据库的DNA样本应常规进行重复扩增。
Zotero 插件