Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
Adv Biochem Eng Biotechnol. 2010;117:85-108. doi: 10.1007/10_2009_16.
The coming of age of whole-cell biosensors, combined with the continuing advances in array technologies, has prepared the ground for the next step in the evolution of both disciplines - the whole cell array. In the present chapter, we highlight the state-of-the-art in the different disciplines essential for a functional bacterial array. These include the genetic engineering of the biological components, their immobilization in different polymers, technologies for live cell deposition and patterning on different types of solid surfaces, and cellular viability maintenance. Also reviewed are the types of signals emitted by the reporter cell arrays, some of the transduction methodologies for reading these signals, and the mathematical approaches proposed for their analysis. Finally, we review some of the potential applications for bacterial cell arrays, and list the future needs for their maturation: a richer arsenal of high-performance reporter strains, better methodologies for their incorporation into hardware platforms, design of appropriate detection circuits, the continuing development of dedicated algorithms for multiplex signal analysis, and - most importantly - enhanced long term maintenance of viability and activity on the fabricated biochips.
全细胞生物传感器的成熟，结合阵列技术的不断进步，为这两个学科的下一步发展——全细胞阵列——奠定了基础。在本章中，我们重点介绍了功能性细菌阵列所需的不同学科的最新技术。这些包括生物成分的遗传工程、它们在不同聚合物中的固定化、在不同类型的固体表面上进行活细胞沉积和图案化的技术，以及细胞活力的维持。我们还回顾了报告细胞阵列发出的信号类型、读取这些信号的一些转换方法，以及为分析这些信号而提出的数学方法。最后，我们回顾了细菌细胞阵列的一些潜在应用，并列出了它们成熟的未来需求：更丰富的高性能报告菌株库、更好的方法将其纳入硬件平台、设计合适的检测电路、继续开发专用算法进行多路复用信号分析，以及——最重要的是——在制造的生物芯片上增强长期的活力和活性维持。
