Energy Environmental Engineering Major, Division of Energy Bioengineering, Dongseo University, Busan 617-716, Republic of Korea.
Appl Microbiol Biotechnol. 2011 Feb;89(4):867-77. doi: 10.1007/s00253-010-2990-8. Epub 2010 Nov 10.
Microbial biosensors are compact, portable, cost effective, and simple to use, making them seem eminently suitable for the in situ monitoring of environmental pollution. One promising approach for such applications is the fusion of reporter genes with regulatory genes that are dose-dependently responsive to the target chemicals or physiological signals. Their biosensor capabilities, such as target range and sensitivity, could be improved by modification of regulatory genes. Recent uses of such genetically engineered microbial biosensors include the development of portable biosensor kits and high-throughput cell arrays on chips, optic fibers, or other platforms for on-site and on-line monitoring of environmental pollution. This mini-review discusses recent advances in microbial biosensors and their future prospects, with a focus on the development and application of genetically modified microbial biosensors for in situ environmental monitoring.
微生物生物传感器具有结构紧凑、携带方便、成本效益高、使用简单等优点，非常适合用于现场监测环境污染。对于此类应用，一种很有前途的方法是将报告基因与调控基因融合，这些调控基因可以根据目标化学物质或生理信号的剂量做出响应。通过修饰调控基因，可以提高其生物传感器的性能，如目标范围和灵敏度。最近，人们利用这种经过基因工程改造的微生物生物传感器，开发了便携式生物传感器试剂盒和高通量细胞微阵列，这些微阵列可以安装在芯片、光纤或其他平台上，用于现场和在线监测环境污染。本综述讨论了微生物生物传感器的最新进展及其未来前景，重点介绍了用于现场环境监测的基因修饰微生物生物传感器的开发和应用。
