Nizhny Novgorod State Medical Academy, 603005 Minin Sq., 10/1, Nizhny Novgorod, Russia.
J Biophotonics. 2010 Oct;3(10-11):718-27. doi: 10.1002/jbio.201000061.
The goal of this study is the development of a method of local laser hyperthermia with gold nanoparticles under noninvasive optical monitoring of nanoparticle accumulation in tumor tissue in vivo. Bifunctional plasmon resonant nanoparticles that are optimal for OCT diagnostics and laser heating at the wavelength of 810 nm were used in the study. The OCT examination showed that the accumulation of gold nanoparticles in the tumor invading into skin was maximal 4-5 h after intravenous injection. It was demonstrated that nanoparticle accumulation in tumor allowed more local heating and enhanced thermal sensitivity of tumor tissue. Laser hyperthermia that heated tumor up to 44-45 °C at maximum nanoparticle accumulation induced apoptotic death of tumor cells and inhibited tumor growth by 104% on the 5th day after treatment.
本研究的目的是开发一种方法，即在非侵入性光学监测下，利用金纳米粒子进行局部激光热疗，以实现肿瘤组织内纳米颗粒的积累。本研究中使用了双功能等离子体共振纳米粒子，其在 OCT 诊断和 810nm 激光加热方面具有最佳性能。OCT 检查显示，金纳米粒子在侵入皮肤的肿瘤中的积累在静脉注射后 4-5 小时达到最大值。结果表明，肿瘤内纳米颗粒的积累允许更局部的加热，并增强了肿瘤组织的热敏感性。激光热疗可将肿瘤加热至 44-45°C，在最大纳米颗粒积累时，可诱导肿瘤细胞凋亡死亡，并在治疗后第 5 天抑制肿瘤生长 104%。
