Aquapri Danmark A/S, Egtved, Denmark.
J Fish Dis. 2010 Sep;33(9):707-16. doi: 10.1111/j.1365-2761.2010.01179.x. Epub 2010 Jul 7.
The effect of two disinfectants on eggs and larvae of Baltic cod, Gadus morhua, was investigated. The eggs were disinfected for 10 min using various concentrations of either glutaraldehyde (100, 200, 400, 600 and 800 mg L(-1)) or iodophor (10, 50, 100 and 150 mg L(-1)), 1-4-days post-fertilization. Bactericidal effect of disinfection, survival to hatching, hatching success and larval abnormalities were assessed. Larval survival was recorded at 5-, 10- and 15-days post-hatch (dph). Although Baltic cod eggs have an unusually thin chorion, they could tolerate surface disinfection. A reduction in bacterial growth was observed with increased concentrations of disinfectant (3.0 x 10(7)-1.6 x 10(1) CFU mL(-1)). Abnormalities in newly hatched larvae were not related to disinfection. Survival of the yolk sac larvae was significantly better for eggs treated with 400 mg L(-1) glutaraldehyde for 10 min at 10 and 15 dph. Effective disinfection was also recorded using 100 mg L(-1) Actomar K30. Egg batch effect rather than initial bacterial concentration, disinfectant type or incubation method determined the survival of the eggs to hatching and survival of larvae. Because of the carcinogenic effect of glutaraldehyde, iodophor is recommended for routine disinfection of cod eggs.
研究了两种消毒剂对波罗的海鳕鱼（ Gadus morhua ）卵和幼虫的影响。在受精后 1-4 天，使用不同浓度的戊二醛（100、200、400、600 和 800mg/L）或碘伏（10、50、100 和 150mg/L）对卵进行 10 分钟的消毒。评估了消毒的杀菌效果、孵化成活率、孵化成功率和幼虫异常情况。幼虫存活率在孵化后 5、10 和 15 天（dph）记录。尽管波罗的海鳕鱼卵的卵壳异常薄，但它们可以耐受表面消毒。随着消毒剂浓度的增加，细菌生长减少（3.0 x 10(7)-1.6 x 10(1) CFU/mL）。新孵化的幼虫的异常情况与消毒无关。用 400mg/L 的戊二醛处理 10 分钟的卵在 10 和 15 dph 时，蛋黄囊幼虫的存活率显著提高。使用 100mg/L 的 Actomar K30 也能有效消毒。卵批效应而不是初始细菌浓度、消毒剂类型或孵化方法决定了卵的孵化成活率和幼虫的成活率。由于戊二醛的致癌作用，建议使用碘伏对鳕鱼卵进行常规消毒。
