Department of Hematology, Cancer Hospital of Tianjin, Tianjin Medical University, Key Laboratory of Cancer Prevention and Therapy, Ti-Yuan-Bei, Huan-Hu-Xi-Road, Tianjin 300060, PR China.
Leuk Res. 2010 Oct;34(10):1325-9. doi: 10.1016/j.leukres.2010.05.006. Epub 2010 Jun 8.
In this study we investigated the influence of the mesenchymal stem cells on the survival of U266 and H929 myeloma cell lines. We found that both soluble factors and direct cell contact inhibited daunorubicin-induced apoptosis of U266 and H929 cells. The mRNA and protein levels of survivin were upregulated in U266 and H929 cells cocultured with mesenchymal stem cells, whereas not increased in that separated by TW insert. Antisense oligonucleotides targeting survivin downregulated the expression of survivin and increased the apoptosis of U266 and H929 cells. The results indicated that MSCs had anti-apoptotic effect on U266 and H929 cells that was mediated by survivin.
在这项研究中，我们研究了间充质干细胞对 U266 和 H929 骨髓瘤细胞系存活的影响。我们发现，无论是可溶性因子还是直接细胞接触，都能抑制 U266 和 H929 细胞中柔红霉素诱导的细胞凋亡。与 TW 插入物分离的细胞不同，与间充质干细胞共培养的 U266 和 H929 细胞中的 survivin mRNA 和蛋白水平上调，而 survivin 的表达水平没有增加。针对 survivin 的反义寡核苷酸下调了 survivin 的表达，并增加了 U266 和 H929 细胞的凋亡。结果表明，间充质干细胞对 U266 和 H929 细胞具有抗凋亡作用，这种作用是通过 survivin 介导的。
