Trimeris, Inc., 3500 Paramount Parkway, Morrisville, NC 27560, USA.
J Pept Sci. 2010 Sep;16(9):465-72. doi: 10.1002/psc.1262.
A helical, prehairpin trimer covering the majority of the HR1 region of human immunodeficiency virus gp41 was achieved by chemically coupling three identical 51 amino acid peptides. A 1,3,5-tris(aminomethyl)-2,4,6-triethylbenzene pinwheel 'cap' was used to trimerize the peptides by taking advantage of the unique property of triacyl fluoride and orthogonal protection and deprotection. The resulting protein is fully helical, highly thermostable and soluble.
通过化学偶联三个相同的 51 个氨基酸肽，实现了覆盖人类免疫缺陷病毒 gp41 的 HR1 区大部分区域的螺旋前发夹三聚体。使用 1,3,5-三(氨甲基)-2,4,6-三乙基苯轮烷“帽”通过利用三酰氟和正交保护和脱保护的独特性质来三聚化肽。得到的蛋白质是完全螺旋的，具有高热稳定性和可溶性。
